LOCUS       sequence-104488-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the
            product of which converts beta-carotene primarily to monocyclic 
            beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Cunningham FX Jr, Gantt E
  TITLE     A study in scarlet: enzymes of ketocarotenoid biosynthesis in the 
            flowers of Adonis aestivalis.
  JOURNAL   Plant J. 2005 Feb;41(3):478-92.
  PUBMED    15659105
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2005-02"; volume: "41(3)"; pages: "478-92"
COMMENT     Sequence Label: pAtCHYb1-Delta-129N
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(689..703,704..712))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(716..748)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(752..769)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(785..811)
                     /codon_start=1
                     /product="synthetic cistron containing a ribosome binding 
                     site (Shine-Dalgarno sequence), for enhancing the bacterial
                     expression of a downstream cistron (Schoner, 1997)"
                     /label=minicistron
                     /note="This first cistron should terminate 3 bp upstream of
                     the ATG for the second cistron."
                     /translation="MYRLNKEE"
ORIGIN
        1 aacagccaag cttcgaattc ccatatggta ccagctgcag atctcgagct cggatcggcc
       61 gcagaccgtc ttaagatgat cataatgaca gaagacaaaa aaagaatttg ggatttaaaa
      121 cttgtttaaa gtcaagaact cgaactcgac ccggagcccg aggccttttt gtatgatttg
      181 attctccgac taatctcctt atctaactct tcatttcctc caacttcttc caattccttg
      241 ggtccaagaa acagtccata tggtacacca ttgaacttgt ctgtgtgatg tagctggtga
      301 gcggcggcga cctttcggag gtaagggacg tcggcgatgg gacctacagg gaaacgcttg
      361 tgcacgagac catcgtggac aaacatgtag gcgattccaa acaccgttat gcctaacccg
      421 gcgccaaagc agagaccagg aacgagtcct ttattgaaga atccataaga gaggagacca
      481 atcgctggac cagcgttcac tatagcaaaa acatcgttta gctcaaacgg tccttctctt
      541 ggtttgtgat gtgactcatg catattccat agagaagcgt gccacagagc tctatgagcc
      601 catcttgccc agaattccat accaacagca gcaccaacag agagagcaaa tgtaccaaac
      661 atttccaaca ttgagatccc catcgatcct tatcgtcatc gtcgtacaga tcccgaccca
      721 tttgctgtcc accagtcatg ctagccatac catgatgatg atgatgatga gaacccccca
      781 tggtttattc ctccttattt aatcgataca ttaatatata cctctttaat t
//
