LOCUS       sequence-10450-e        3863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Lynch MD, Gill RT
  TITLE     Broad host range vectors for stable genomic library construction.
  JOURNAL   Biotechnol Bioeng. 2006 May 5. 94(1):151-8.
  PUBMED    16496398
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2006 May 5. 94(1):151-8."
COMMENT     Sequence Label: pBMTL-2
FEATURES             Location/Qualifiers
     source          1..3863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(60..96)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     rep_origin      1347..2116
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             2117..2779
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     CDS             2876..3691
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2949..2968)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(3708..3739)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        join(3786..3791,3792..3809,3810..3816)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3824..3840
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt
      121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct
      181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct
      241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa
      301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc
      361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt
      421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt
      481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt
      541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct
      601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct
      661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct
      721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg
      781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga
      841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt
      901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc
      961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt
     1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca
     1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt
     1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg
     1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc
     1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc
     1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt
     1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag
     1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc
     1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc
     1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg
     1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc
     1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt
     1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc
     1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg
     1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa
     1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc
     1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt
     2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta
     2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa
     2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc
     2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa
     2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt
     2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg
     2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga
     2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga
     2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca
     2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc
     2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc
     2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt
     2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc
     2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgag
     2881 ccatattcaa cgggaaacgt cttgctcgag gccgcgatta aattccaaca tggatgctga
     2941 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     3001 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     3061 caatgatgtt acagatgaga tggtcaggct aaactggctg acggaattta tgcctcttcc
     3121 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     3181 agggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     3241 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     3301 cggcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttgg
     3361 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     3421 gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     3481 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     3541 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     3601 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     3661 gtttcacttg atgctcgatg agtttttctg agggcggatc cccctcaagt caaaagcctc
     3721 cggtcggagg cttttgactt tctgctatgg aggtcaggta tgattttgca ttaggcaccc
     3781 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3841 tttcacactc tagagaattc gtc
//
