LOCUS       sequence-104507-        5913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HSPB10 (small HSP) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, 
            Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH
  TITLE     HSPB7 is the most potent polyQ aggregation suppressor within the 
            HSPB family of molecular chaperones.
  JOURNAL   Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. 
            Epub 2010 Sep 15.
  PUBMED    20843828
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddq398"; journalName: "Hum Mol Genet"; date: 
            "2010-12-1- 1"; volume: "19"; issue: "23"; pages: "4677-93"
COMMENT     Sequence Label: Frt-hspB10
FEATURES             Location/Qualifiers
     source          1..5913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(858..875)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    864..1088
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1221..1240)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    1372..1419
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1427..2449
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             1427..2449
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    2579..2700
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2616..2635)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2670..2689
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2749..2765)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2749..2765)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2762..2784)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2773..2789
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2797..2803,2804..2821,2822..2827))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2842..2863
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2980..2997)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3151..3739)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3231..3250)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3910..4701,4702..4770))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4533..4552
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4771..4875)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4950..4969)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5141..5520
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5521..5724
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5675..5695
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    5726..5744
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5747..5765
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     5775..5799
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tcgagaacca tggctgcact gagttgtctc ttggacagtg tcagaaggga cataaagaag
       61 gtggacagag aactaaggca actgagatgc atcgacgaat ttagcacacg gtgcctgtgc
      121 gacttgtata tgcaccccta ttgctgctgt gacttgcacc catatccgta ctgcttgtgc
      181 tattccaagc gatcacgctc ttgcggcctg tgtgatctct acccatgttg cctgtgtgat
      241 tataagcttt actgtctgcg accatctctc agaagtttgg agaggaaagc catcagagcc
      301 atagaagatg agaagcgaga gcttgccaaa ctgagaagaa caacaaatag aattctggct
      361 tcctcctgct gtagcagtaa cattttagga tcggtgaatg tatgcggttt tgaacccgat
      421 caagtcaaag ttcgagtgaa ggatggaaag gtatgtgtgt cggctgagcg ggagaacagg
      481 tacgactgcc ttggatcgaa aaagtacagc tacatgaaca tctgcaaaga gttcagcttg
      541 ccgccctgtg tggatgagaa ggatgtaaca tactcctatg ggctcggcag ctgtgtcaag
      601 atcgagtctc cttgctaccc ttgcacttct ccttgcagcc cgtgcagccc gtgcaacccc
      661 tgcaacccct gcagcccctg caacccgtgc agcccatatg atccttgcaa cccgtgttat
      721 ccctgtggaa gccgattttc ctgtaggaag atgattttgt aaagtgcgca taggaaccca
      781 ttacttaata gaagtcagtt actccagcca acgcgccaaa atcgagtcta gagggcccgt
      841 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
      901 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
      961 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     1021 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     1081 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     1141 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     1201 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     1261 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     1321 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtacct agaagttcct
     1381 attccgaagt tcctattctc tagaaagtat aggaacttcc ttggccaaaa agcctgaact
     1441 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     1501 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     1561 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     1621 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag
     1681 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     1741 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     1801 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     1861 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     1921 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     1981 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     2041 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     2101 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     2161 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     2221 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     2281 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     2341 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     2401 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagc acgtactacg
     2461 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     2521 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     2581 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     2641 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     2701 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     2761 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     2821 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     2881 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     2941 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3001 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3061 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3121 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3181 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3241 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3301 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3361 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3421 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3481 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3541 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3601 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3661 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3721 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3781 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3841 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3901 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3961 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4021 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4081 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4141 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4201 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4261 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4321 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4381 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4441 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4501 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4561 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4621 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4681 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4741 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4801 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4861 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     4921 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     4981 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     5041 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     5101 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
     5161 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     5221 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     5281 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     5341 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     5401 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     5461 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     5521 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     5581 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     5641 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     5701 tgggaggtct atataagcag agctctccct atcagtgata gagatctccc tatcagtgat
     5761 agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     5821 tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct agcgtttaaa
     5881 cttaagcttg gtaccgagct cggatccaaa acc
//
