LOCUS       sequence-104524-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pET23a_mod_RBPMS_FL
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     207..226
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        207..225
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             257..279
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             287..319
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             335..358
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             359..385
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc
       61 ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc
      121 ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc
      181 tgcccgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
      241 tccctctaga aataattttg tttaacttta agaaggagat atacatatgg ctagcatgac
      301 tggtggacag caaatgggtc gcggatcgac cggtgactac aaggacgacg atgacaagta
      361 cccttatgac gtgcccgatt acgctggatc cgaattcgag ctccgtcgac ccatgaacaa
      421 cggcggcaaa gccgagaagg agaacacccc gagcgaggcc aaccttcagg aggaggaggt
      481 ccggacccta tttgtcagtg gccttcctct ggatatcaaa cctcgggagc tctatctgct
      541 tttcagacca tttaagggct atgagggttc tcttataaag ctcacatcta aacagcctgt
      601 aggttttgtc agttttgaca gtcgctcaga agcagaggct gcaaagaatg ctttgaatgg
      661 catccgcttc gatcctgaaa ttccgcaaac actacgacta gagtttgcta aggcaaacac
      721 gaagatggcc aagaacaaac tcgtagggac tccaaacccc agtactcctc tgcccaacac
      781 tgtacctcag ttcattgcca gagagccata tgagctcaca gtgcctgcac ttt
//
