LOCUS       sequence-104525-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pET23a_mod_RBPMS_FL
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..55
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(108..125)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(756..782)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(783..806)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cccctcaaga cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca
       61 gcagccaact cagcttcctt tcgggctttg ttagcagccg gatctcagtg gtggtggtgg
      121 tggtgctcga gtgcggccgc gcagaactga cgggacttcc agccctgaga agtagcctcg
      181 gagggaggga gccagcgcat ctgggcatgc agtgaagcgg gataggtgaa agcaggagga
      241 ggtagagcag gcgctaactc cgctgggtac agagggtacg gggcccacac ttcagggcta
      301 ctggggtaaa gtgcaggcac tgtgagctca tatggctctc tggcaatgaa ctgaggtaca
      361 gtgttgggca gaggagtact ggggtttgga gtccctacga gtttgttctt ggccatcttc
      421 gtgtttgcct tagcaaactc tagtcgtagt gtttgcggaa tttcaggatc gaagcggatg
      481 ccattcaaag cattctttgc agcctctgct tctgagcgac tgtcaaaact gacaaaacct
      541 acaggctgtt tagatgtgag ctttataaga gaaccctcat agcccttaaa tggtctgaaa
      601 agcagataga gctcccgagg tttgatatcc agaggaaggc cactgacaaa tagggtccgg
      661 acctcctcct cctgaaggtt ggcctcgctc ggggtgttct ccttctcggc tttgccgccg
      721 ttgttcatgg gtcgacggag ctcgaattcg gatccagcgt aatcgggcac gtcataaggg
      781 tacttgtcat cgtcgtcctt gtagtcaccg gtcgatccgc gacccatttg ctg
//
