LOCUS       sequence-104526-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for bacterial overexpression of RBPMS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pET23a_mod_RBPMS2_FL
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     195..214
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        195..213
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             245..267
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             275..307
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             323..346
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             347..373
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct
       61 cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc
      121 tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatct
      181 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaaa
      241 taattttgtt taactttaag aaggagatat acatatggct agcatgactg gtggacagca
      301 aatgggtcgc ggatcgaccg gtgactacaa ggacgacgat gacaagtacc cttatgacgt
      361 gcccgattac gctggatccg aattcgagct ccgtcgaccc atgagcaacc tgaagccgga
      421 cggcgagcac ggcggcagca ccggcaccgg ctccggcgcg ggctccggcg gcgccctgga
      481 ggaggaggtc cggacactgt ttgtcagcgg cctccctgtg gacattaaac ccagagaact
      541 ctacttgctc ttccggccgt tcaaggggta tgaagggtcc ctgatcaagc tcactgcaag
      601 acagcctgtt ggttttgtga tctttgacag ccgtgcagga gcagaagcgg ccaagaatgc
      661 gctgaacggt attcgctttg atcccgaaaa tccacagact ctgaggctag agtttgccaa
      721 agccaacacc aagatggcca agagcaagct aatggcaact ccaaatccca gcaacgtgca
      781 ccccgcccta ggagcacact tcatcgcacg ggacccctat gacctgatgg gggctgctct
      841 gatccctgca tccccagagg cc
//
