LOCUS       sequence-104528-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector with RBPMS2 for stable cell line generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS2
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    106..330
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     319..336
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(416..457)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    510..534
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cggttacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
       61 ggggataccc cctagagccc cagctggttc tttccgcctc agaagccata gagcccaccg
      121 catccccagc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc
      181 caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt
      241 aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa
      301 caacagatgg ctggcaacta gaaggcacag tcgaggctga tcagcgggtt taaacgggcc
      361 ctctagactc gaggcacact ggatcagtta tctatgcggc cgcggatcag gtacgcgtag
      421 aatcgagacc gaggagaggg ttagggatag gcttaccttc gaaccgcggg ccctctagac
      481 ttgagcggcc gccactgtgc tggatatcaa ccactttgta caagaaagct gggtgtcaac
      541 agaactgacg gtacttccat ccttgctggg tggtgtcaga ggaagggtac cagcgcacct
      601 gagcgtggag ggcggcggca gcggcagtgg cagttgggta ggtgaacgca gcatgggaga
      661 tggctggggt cagctctgtg gtgtacaaag ggtagggggc ccaggcctct ggggatgcag
      721 ggatcagagc agcccccatc aggtcatagg ggtcccgtgc gatgaagtgt gctcctaggg
      781 cggggtgcac gttgctggga tttggagttg ccattagctt gctcttggcc
//
