LOCUS       sequence-104529-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector with RBPMS2 for stable cell line generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS2
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     60..84
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             184..207
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             208..234
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    250..274
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
       61 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      121 agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa gcttggtacc
      181 atggactaca aggacgacga tgacaagtac ccttatgacg tgcccgatta cgctttgaac
      241 cagatatcaa caagtttgta caaaaaagca ggcttcatga gcaacctgaa gccggacggc
      301 gagcacggcg gcagcaccgg caccggctcc ggcgcgggct ccggcggcgc cctggaggag
      361 gaggtccgga cactgtttgt cagcggcctc cctgtggaca ttaaacccag agaactctac
      421 ttgctcttcc ggccgttcaa ggggtatgaa gggtccctga tcaagctcac tgcaagacag
      481 cctgttggtt ttgtgatctt tgacagccgt gcaggagcag aagcggccaa gaatgcgctg
      541 aacggtattc gctttgatcc cgaaaatcca cagactctga ggctagagtt tgccaaagcc
      601 aacaccaaga tggccaagag caagctaatg gcaactccaa atcccagcaa cgtgcacccc
      661 gccctaggag cacacttcat cgcacgggac ccctatgacc tgatgggggc tgctctgatc
      721 cctgcatccc cagaggcctg ggccccctac cctttgtaca ccacagagct gaccccagcc
      781 atctcccatg ctgcgttcac ctacccaact gccactgccg ctgccgccgc cctcca
//
