LOCUS       sequence-104530-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of tgRFPt-Micro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear 
            JE, Kuhlman B
  TITLE     Engineering an improved light-induced dimer (iLID) for controlling 
            the localization and activity of signaling proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 
            10.1073/pnas.1417910112. Epub 2014 Dec 22.
  PUBMED    25535392
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1417910112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-01-6- 6"; volume: "112"; issue: "1"; pages: "112-7"
COMMENT     Sequence Label: pLL7.0: tgRFPt-SSPB R73Q
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        75..387
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     306..326
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     403..419
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     404..420
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(462..495)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
       61 taccgcgctc tagagatccg acgcgccatc tctaggcccg cgccggcccc ctcgcacaga
      121 cttgtgggag aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag
      181 taactataga ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct
      241 acattttaca tgataggctt ggatttctat aagagataca aatactaaat tattatttta
      301 aaaaacagca caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat
      361 aaatatccct tggagaaaag ccttgttaac gcgcggtgac cctcgaggtc gacggtatcg
      421 ataagctcgc ttcacgagat tccagcaggt cgagggacct aataacttcg tatagcatac
      481 attatacgaa gttatattaa gggttccaag cttaagcggc cgcgtggata accgtattac
      541 cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      601 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      661 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      721 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      781 atcatatgcc aagtacgccc cctattga
//
