LOCUS       sequence-104531-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of tgRFPt-Micro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear 
            JE, Kuhlman B
  TITLE     Engineering an improved light-induced dimer (iLID) for controlling 
            the localization and activity of signaling proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 
            10.1073/pnas.1417910112. Epub 2014 Dec 22.
  PUBMED    25535392
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1417910112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-01-6- 6"; volume: "112"; issue: "1"; pages: "112-7"
COMMENT     Sequence Label: pLL7.0: tgRFPt-SSPB R73Q
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    99..132
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(187..306,307..309,310..516))
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein and with the mutation R74Q to reduce the 
                     affinity for iLID"
                     /label=SspB micro
                     /note="dimerizes with iLID at moderate affinity to create 
                     iLID micro (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNAQFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
ORIGIN
        1 taccagtcaa tctttcacaa attttgtaat ccagaggttg attatcgata agcttgatat
       61 cgaattgtac ctagtggaac cggaaccctt aaacatgtat aacttcgtat aatgtatgct
      121 atacgaagtt attaggtccc tcgacgaatt gcctgcaggt tatcttgatc cggtggatcc
      181 ttaaccaata ttcagctcgt catagatttc ttctggttcg aacatcacac catcgccgtt
      241 ctcgcgagcg taaatggcca gagcggcacc catcgggata tacagttcac gagacacgcc
      301 cttaaactgg gcgttgaact ggataaaatc atttgtcagt tgcaggttgc cggtcgcact
      361 tgcagacaga ttcagcacga tctgaccgtc tttcacatac tccacgggca cgttcacgcc
      421 caggtatgtg gcatccacca ccagatatgg ggtaaagctg ttatcaacca gccaatcgta
      481 atattcacgc agcagcttag ggcgtttcgg ggagctgaat tcctgcaggg tcaggccgct
      541 cagatcaatg ccgaattcgt tcgaagcttg agctcgagat ctgagtccgg acttgtacag
      601 ctcgtccatg ccattaagtt tgtgccccag tttgctaggg aggtcgcagt atctggccac
      661 agccacctcg tgctgctcga cgtaggtctc tttgtcggcc tccttgattc tttccagtct
      721 gtggtccaca tagtagacgc cgggcatctt gaggttctta gcgggtttct tggatctgta
      781 tgtggtcttg aagttgcaga tca
//
