LOCUS       sequence-10454-e        5922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Lynch MD, Gill RT
  TITLE     Broad host range vectors for stable genomic library construction.
  JOURNAL   Biotechnol Bioeng. 2006 May 5. 94(1):151-8.
  PUBMED    16496398
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2006 May 5. 94(1):151-8."
COMMENT     Sequence Label: pBMTL-6
FEATURES             Location/Qualifiers
     source          1..5922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(60..96)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     rep_origin      1347..2116
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             2117..2779
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     terminator      complement(5767..5798)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        join(5845..5850,5851..5868,5869..5875)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5883..5899
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt
      121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct
      181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct
      241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa
      301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc
      361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt
      421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt
      481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt
      541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct
      601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct
      661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct
      721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg
      781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga
      841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt
      901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc
      961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt
     1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca
     1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt
     1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg
     1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc
     1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc
     1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt
     1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag
     1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc
     1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc
     1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg
     1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc
     1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt
     1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc
     1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg
     1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa
     1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc
     1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt
     2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta
     2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa
     2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc
     2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa
     2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt
     2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg
     2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga
     2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga
     2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca
     2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc
     2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc
     2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt
     2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc
     2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga
     2881 agaacaaagc gtgaacatgg cgcgattgaa gggggaggtt ttgcccgccc tcttcgcgtc
     2941 gccggcgacg attggcgagt acggggccgg catcgacggg gcggattccc tcaacgagct
     3001 gtcgaatctg atggagcacg gcgcagttgc cgcgctggcc gacaaaatca gccagatcgt
     3061 ggcgaagctg gccgacgcgg acccccgcaa gatcgcggaa aagcctacct ggttcgagaa
     3121 gatgcttggc cgtgaggttg aacgccaggt gaggtatcag gtcgcccgca agacgctcga
     3181 ccagttgctg gacgaagccg agggcgtagc gcagcgcgtg cgggacacgt tgcgcgcctt
     3241 ggatgacatg ctcaatacgc atgaggccga ggtagaccgg ctcagagcct acattcaagc
     3301 cgggcgcgag ttcctggacg agaaccccga ggccggcgcg gccaaggccg gcgtgatcga
     3361 gttcgacaag ccgcgcgaac gcttcgcgcg caagctcgcc aacctggcaa ccctcatggc
     3421 gtcccatgaa atgagcgtca ctcagatgaa gctcacgcgg gcgcaggccg tggacatgct
     3481 ggaccgcttc tctgaaacgg catccgtcct ggtgcccgtc tggcgtcagc acaccctcgc
     3541 gctcatcacc accaagaaca tgaatccggc aatggtcgcc gaggcggcca aagctcacca
     3601 ggcgctcatg cggagccttt cgcagagcct ggaaggcatc aaccaataac acggcgggag
     3661 aaccctatga acgcactgaa aacgacgcac gacgccaagg cccctatcgt cgccttcgac
     3721 atgaccccgg caaccctgcg cgagctgggc ttgcaggaaa gcgacgtgcc ggaagtccat
     3781 gcggtcgcgc agcggatcga ggtcggcagt ccgcagaccg ttgccgagtt cggccgcgac
     3841 gtggccgagc acacgtcccg ctacgccgat agcctgctgg accaggtgcg caacagcgac
     3901 ctggacgaag caggcgagaa actgacccag gttgtcgcca aggcccgttc cctgaacgtc
     3961 ggccctttgt ccgacaaccg ttcccgcctg cccctgattg gcccgctgat cgaccgcttc
     4021 cgcgtccgtt cgacgggctt catggcgcgc ttcgacacga cccgcgagca gatcgaacac
     4081 ctggtcagcg aagtgcagac cacccagcaa ggcatcgcgc agcgcaatgc ctcgctcgac
     4141 gaaatgttcg cagccgtgcg cgaggaacac cgccttcttg gcgtccacat cgcggccggc
     4201 aaggtccgcc ttgccgagct gcgcgagcag gccgagggtc tgcgcggcaa tgtcgggaac
     4261 gacccgggcc gcgtgcagga gctggccgac ctcgatgcga tggttgccaa cctggacaag
     4321 cgcatcggcg acctgatcgc cttgcaacat tcggccatgc agagcctgcc gaccatccgc
     4381 atgatccagg ccaacaacca gatgctggtc gataaattcc acaccatccg cgaaatcacc
     4441 gtgccggcgt ggaagcggca attcatgctg gccttgagcc tcaacgagca gaagaacgcc
     4501 gtcgaactgg ccacggccat cgacgacacc accaacgacc tgatgaagcg caatgcggcc
     4561 ctgctgcatc gcacgtccgt cgagacggcg aaggagaacc aacgcctggt gatcgacgtg
     4621 gacacgctca agcaggttca gacgacgctc atcaagaccg tcgaggacgt tattcgcatc
     4681 cagcaggaag gcgtgcagaa gcgcaaggat gccgagaagc agatcgccgc aatgcgtggc
     4741 gatcttcaag ccaagctgac ccgccagccc gtgcgcgagc tggcccaaca ggagtccgta
     4801 tgaatgccac aaacaccgat gttttcgccc aggtaggcgg cctcgaggcc cgaggcgcga
     4861 agatgaagaa gcggggcacc cgcttcctca tcgcggcgct ggcagtcctt gccattgccg
     4921 ggatcggggc agtaacggga tgggcgatca gcccgagcgc gacgcccgga agcattgacg
     4981 tgccgcaggt gctggcatcg acattcagcg accaggtgcc gggcagtgag ggcggcggcc
     5041 tgggtggcgg cctgcccttc acttcggccg tcggggcatt cacggacttc atggcggggc
     5101 cggcaatttt taccttgggc attcttggca tagtggtcgc gggtgccgtg ctcgtgttcg
     5161 ggggtgaatt ctgcgggttc gtgcgatccg tctgcatgat ggtgatagcc gtcagcatga
     5221 ttttcgtgtc gtcgaacttg gtgaagggca ttctcggcgg cgatcacgac gccggccctg
     5281 cggagccttc gccgcgtgcg cgattcatgg cggccgtgga ggccaaggat ttcgcgcgag
     5341 tgcaagagct gatcgaggcg cgtggagcca agtcggcggc tgattatgtc cttgcgcagc
     5401 tcgccgtggc cgaaggtctg gaccgcaagc ctggtgcgcg cgtcgtggtc gggaaagcgg
     5461 cgggcagcat ggcaatgccg cctgcggcgc tgggttttac gccaagggga gaagcggcat
     5521 acgccatcga gcggtcagcc tatggtgagc cgaggtccag cattgcgaag cagtaccagc
     5581 aggaatggaa ccggaaggcg gcgacctggt gggcgatggc cggtgtggcc ggcatcatcg
     5641 gcgcgatcct ggcggcggcg gcaaccggct ttgttgggct ggcagtgtcg atccgcaacc
     5701 gagtgaagcg cgtgcgcgac ctgttggtga tggagccggg tgcagagcca taagggatcc
     5761 ccctcaagtc aaaagcctcc ggtcggaggc ttttgacttt ctgctatgga ggtcaggtat
     5821 gattttgcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     5881 aattgtgagc ggataacaat ttcacactct agagaattcg tc
//
