LOCUS       sequence-104542-        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter containing the 3'UTR of mouse Crtap with MRE-1 
            mutated.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Perdigao-Henriques R, Petrocca F, Altschuler G, Thomas MP, Le MT, 
            Tan SM, Hide W, Lieberman J
  TITLE     miR-200 promotes the mesenchymal to epithelial transition by 
            suppressing multiple members of the Zeb2 and Snail1 transcriptional 
            repressor complexes.
  JOURNAL   Oncogene. 2015 Mar 23. doi: 10.1038/onc.2015.69.
  PUBMED    25798844
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2015 Mar 23. doi: 10.1038/onc.2015.69."
COMMENT     Sequence Label: psiCHECK-2-Crtap-3'UTR-MRE-1 MUT
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      66..75
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             1031..1039
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ctcgaacacc gagcgaccct gcagcgaccc gcttaaaagc ttggcattcc ggtactgttg
       61 gtaaagccac catggccgat gctaagaaca ttaagaaggg ccctgctccc ttctaccctc
      121 tggaggatgg caccgctggc gagcagctgc acaaggccat gaagaggtat gccctggtgc
      181 ctggcaccat tgccttcacc gatgcccaca ttgaggtgga catcacctat gccgagtact
      241 tcgagatgtc tgtgcgcctg gccgaggcca tgaagaggta cggcctgaac accaaccacc
      301 gcatcgtggt gtgctctgag aactctctgc agttcttcat gccagtgctg ggcgccctgt
      361 tcatcggagt ggccgtggcc cctgctaacg acatttacaa cgagcgcgag ctgctgaaca
      421 gcatgggcat ttctcagcct accgtggtgt tcgtgtctaa gaagggcctg cagaagatcc
      481 tgaacgtgca gaagaagctg cctatcatcc agaagatcat catcatggac tctaagaccg
      541 actaccaggg cttccagagc atgtacacat tcgtgacatc tcatctgcct cctggcttca
      601 acgagtacga cttcgtgcca gagtctttcg acagggacaa aaccattgcc ctgatcatga
      661 acagctctgg gtctaccggc ctgcctaagg gcgtggccct gcctcatcgc accgcctgtg
      721 tgcgcttctc tcacgcccgc gaccctattt tcggcaacca gatcatcccc gacaccgcta
      781 ttctgagcgt ggtgccattc caccacggct tcggcatgtt caccaccctg ggctacctga
      841 tttgcggctt tcgggtggtg ctgatgtacc gcttcgagga ggagctgttc ctgcgcagcc
      901 tgcaagacta caaaattcag tctgccctgc tggtgccaac cctgttcagc ttcttcgcta
      961 agagcaccct gatcgacaag tacgacctgt ctaacctgca cgagattgcc tctggcggcg
     1021 ccccactgtc taaggaggtg ggcgaagccg tggccaagcg ctttcatctg ccaggcatcc
     1081 gccagggcta cggcctgac
//
