LOCUS       sequence-104557-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Duet plasmid for weak split mCherry assembly (as compared to 
            N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Sawyer N, Gassaway BM, Haimovich AD, Isaacs FJ, Rinehart J, Regan L
  TITLE     Designed Phosphoprotein Recognition in Escherichia coli.
  JOURNAL   ACS Chem Biol. 2014 Nov 21;9(11):2502-7. doi: 10.1021/cb500658w. 
            Epub 2014 Oct 6.
  PUBMED    25272187
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb500658w";
            journalName: "ACS Chem Biol"; date: "2014-11-21- 21"; volume: "9"; 
            issue: "11"; pages: "2502-7"
COMMENT     Sequence Label: pNAS1B split mCherry N-MESVD/TRAP(RK)-C
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(740..757)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 actctctact gtttctccat acccgttttt tgggctaaca ggaggaatga cgtcatggct
       61 aagcaggcac tgaaagaaaa agagctgggg aacgatgcct acaagaagaa agactttgac
      121 acagccttga agcattacga caaagccaag gagctggacc ccactaacat gacttacatt
      181 accaatcaag cagcggtata ctttgaaaag ggcgactaca ataagtgccg ggagctttgt
      241 gagaaggcca ttgaagtggg gagagaaaac cgagaagact atcgacagat tgccaaagca
      301 tatgctcgaa ttggcaactc ctacttcaaa gaagaaaagt acaaggatgc catccatttc
      361 tataacaagt ctctggcaga acaccgacgc ccaaaagtgc tcaagaaatg ccagcaggca
      421 gagaaaatcc tgaaggagca agcggccgca ggtggaagcg gtggcgccct gaagggcgag
      481 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
      541 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
      601 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
      661 cactccaccg gcggcatgga cgagctgtac aagtgataag agctcttggc tgttttggcg
      721 gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa
//
