LOCUS       sequence-104564-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Positive control duet plasmid for SepOTS-dependent split mCherry 
            assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS
            = phosphoserine); C-mCherry is fused to TRAP(RK)).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Sawyer N, Gassaway BM, Haimovich AD, Isaacs FJ, Rinehart J, Regan L
  TITLE     Designed Phosphoprotein Recognition in Escherichia coli.
  JOURNAL   ACS Chem Biol. 2014 Nov 21;9(11):2502-7. doi: 10.1021/cb500658w. 
            Epub 2014 Oct 6.
  PUBMED    25272187
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb500658w";
            journalName: "ACS Chem Biol"; date: "2014-11-21- 21"; volume: "9"; 
            issue: "11"; pages: "2502-7"
COMMENT     Sequence Label: pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(753..770)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 gctttttatc gcaactctct actgtttctc catacccgtt ttttgggcta acaggaggaa
       61 tgacgtcatg gctaagcagg cactgaaaga aaaagagctg gggaacgatg cctacaagaa
      121 gaaagacttt gacacagcct tgaagcatta cgacaaagcc aaggagctgg accccactaa
      181 catgacttac attaccaatc aagcagcggt atactttgaa aagggcgact acaataagtg
      241 ccgggagctt tgtgagaagg ccattgaagt ggggagagaa aaccgagaag actatcgaca
      301 gattgccaaa gcatatgctc gaattggcaa ctcctacttc aaagaagaaa agtacaagga
      361 tgccatccat ttctataaca agtctctggc agaacaccga cgcccaaaag tgctcaagaa
      421 atgccagcag gcagagaaaa tcctgaagga gcaagcggcc gcaggtggaa gcggtggcgc
      481 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
      541 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
      601 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
      661 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtgat aagagctctt
      721 ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga
      781 agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc
      841 atgccgaact cagaagtgaa acgccgta
//
