LOCUS       sequence-104565-         632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for F-actin LifeAct-mCherry expressed under 
            MtBCP1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMB-ACTLr
FEATURES             Location/Qualifiers
     source          1..632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    161..185
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(462..481)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 taagggtttc ttatatgctc aacacagtga gcgaaaccct ataagaaccc taattccctt
       61 atctgggaac tactcacaca ttattctgga gaaaaataga gagagataga nttgtagaga
      121 gagactggtg atttttgcgg actctagcat ggccgcttat tccactttgt acaagaaagc
      181 tgggtccttg tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta
      241 ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt
      301 gtaggcgccg ggcagctgca cgggcttctt ggccttgtag gtggtcttga cctcagcgtc
      361 gtagtggccg ccgtccttca gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc
      421 ctcggggtac atccgctcgg aggaggcctc ccagcccatg gtcttcttct gcattacggg
      481 gccgtcggag gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg
      541 cagggaggag tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc
      601 ccacttgaaa gccctcgggg aaggacagct tc
//
