LOCUS       sequence-104569-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes an inert blue fluorescent protein for use in oxidizing 
            cellular environments including the periplasm and mammalian 
            secretory pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Costantini LM, Subach OM, Jaureguiberry-bravo M, Verkhusha VV, Snapp
            EL
  TITLE     Cysteineless non-glycosylated monomeric blue fluorescent protein, 
            secBFP2, for studies in the eukaryotic secretory pathway.
  JOURNAL   Biochem Biophys Res Commun. 2013 Jan 18;430(3):1114-9. doi: 
            10.1016/j.bbrc.2012.12.028. Epub 2012 Dec 19.
  PUBMED    23257162
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2012.12.028"; journalName: "Biochem Biophys Res 
            Commun"; date: "2013-01-18- 18"; volume: "430"; issue: "3"; pages: 
            "1114-9"
COMMENT     Sequence Label: psecBFP2
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(121..816)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="ELIKENMHMKLYMEGTVDNHHFKSTSEGEGKPYEGTQTMRIKVVE
                     GGPLPFAFDILATSFLYGSKTFIDHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQ
                     DTSLQDGSLIYNVKIRGVDFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLV
                     GGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANDETYVEQHEVAVARYSDL
                     PSKLGHKLN"
ORIGIN
        1 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
       61 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag tcgcggccgc
      121 ttaattaagc ttgtgcccca gtttgctagg gaggtcgctg tatctggcca ctgccacctc
      181 gtgctgctcg acgtaggtct cgtcgttggc ctccttgatt ctttccagtc tgtagtccac
      241 atagtagacg ccaggcatct tgaggttctt agcgggtttc ttggatctat atgtggtctt
      301 gatgtttgcg atcagatggc tcccgcccac gagcttcagg gccatgtcgt ttctgccttc
      361 caggccgccg tcagcggggt acagcgtctc ggtgaaggcc tcccagccga gtgttttctt
      421 ctgcatcaca gggccgttgg atgtgaagtc cacccctctg atcttgacgt tgtagatgag
      481 gctgccgtcc tggaggctgg tgtcctgggt agcggtcagc acgcccccgt cttcgtatgt
      541 ggtgactctc tcccatgtga agccctcagg gaaggactgc ttgaagaagt cggggatgcc
      601 ctgggtgtgg tcgatgaagg tcttgctgcc gtagaggaag ctagtagcca ggatgtcgaa
      661 ggcgaagggg agagggccgc cctcgaccac cttgattctc atggtctggg tgccctcgta
      721 gggcttgcct tcgccctcgg atgtgctctt gaagtgatgg ttgtccacgg tgccctccat
      781 gtacagcttc atgtgcatgt tctccttaat cagctcctcg ctgctggcga ccggtggatc
      841 ccgggcccgc ggtaccgtcg actgca
//
