LOCUS       sequence-104576-         359 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (AtU6-26 promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHSE401
FEATURES             Location/Qualifiers
     source          1..359
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(83..129)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(118..138)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 tttttcatat ctcattgccc cccggatctg cgaaagctcg agagagatag atttgtagag
       61 agagactggt gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa
      121 gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat
      181 caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg
      241 ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct
      301 ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaa
//
