LOCUS       sequence-104579-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mutant of hamster BiP-mGFP unable to bind clients..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Lai CW, Aronson DE, Snapp EL
  TITLE     BiP availability distinguishes states of homeostasis and stress in 
            the endoplasmic reticulum of living cells.
  JOURNAL   Mol Biol Cell. 2010 Jun 15;21(12):1909-21. doi: 
            10.1091/mbc.E09-12-1066. Epub 2010 Apr 21.
  PUBMED    20410136
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-12-1066"; journalName: "Mol Biol Cell"; date: 
            "2010-06-15- 15"; volume: "21"; issue: "12"; pages: "1909-21"
COMMENT     Sequence Label: BiP-mGFP P495L
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(133..843,844..846,847..849))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(175..196)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     524..543
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     783..804
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      846..855
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
       61 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag tcgcggccgc
      121 ttacaattca tccttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
      181 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcagct tggactgggt
      241 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
      301 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac
      361 cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta
      421 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
      481 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
      541 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
      601 gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt
      661 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
      721 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
      781 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
      841 gctcaccatg gtggcgaccg gtggatcc
//
