LOCUS       sequence-104581-         501 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (AtU6-26 promoter), Kan resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pKSE401
FEATURES             Location/Qualifiers
     source          1..501
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(37..382)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(74..94)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact
       61 tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc
      121 agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
      181 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
      241 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
      301 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
      361 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
      421 cttggagtag acgagagtgt cgtgctccac catgttcaca tcaatccact tgctttgaag
      481 acgtggttgg aacgtcttct t
//
