LOCUS       sequence-104612-        9272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lys2 marker pXR vector uses for both plasmid assembly in vivo, as 
            well as targeted genomic integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Chou CC, Patel MT, Gartenberg MR
  TITLE     A series of conditional shuttle vectors for targeted genomic 
            integration in budding yeast.
  JOURNAL   FEMS Yeast Res. 2015 May;15(3):fov010. doi: 10.1093/femsyr/fov010. 
            Epub 2015 Mar 2.
  PUBMED    25736914
REFERENCE   2  (bases 1 to 9272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2015-05"; volume: "15(3):fov010. doi"; pages: " 
            10.1093/femsyr/fov010. Epub 2015 Mar 2"
COMMENT     Sequence Label: pXRLy2
FEATURES             Location/Qualifiers
     source          1..9272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     106..125
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(526..4704)
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     promoter        complement(4705..4944)
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     rep_origin      complement(5001..5456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5138..5159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5350..5369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(5433..5813)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     5582..5604
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5596..5613
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5597..5613
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5623..5642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5623..5641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    5650..5757
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     5666..5682
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5667..5683
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5717..5733)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5717..5733)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5770..5790)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5770..5788)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5809..5825)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5809..5825)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5822..5844)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5833..5849
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5857..5863,5864..5881,5882..5887))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5902..5923
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6040..6057)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6211..6799)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6291..6310)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6970..7761,7762..7830))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7593..7612
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7831..7922)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    7957..7990
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    8565..8598
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
       61 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
      121 ttgtactgag agtgcaccat agatcagctt cgcaagtatt cattttagac ccatggtgga
      181 accctagtgt tgaatggcaa agtggtgata gagttcatag aattggtcag tatcgacctg
      241 tgaagatcac aaggttttgc attgaggata gtatagaagc aagaatcatt gaattacagg
      301 aaaaaaaggc aaatatgatt catgctacaa taaaccaaga tgaagctgcc attagcagac
      361 taacgccagc tgatttacag ttcttattca ataactaata ttttattctc ttattatata
      421 ttattctcgg agtttttaag tgacatcacc cgaaaagaag ctaagtcttt ctcctaattc
      481 atatttaatt attgtacatg gacatatcat acgtaatgct caaccttaag ctgctgcgga
      541 gcttccacga gcaccagcac ctgaagcaac tagacttatt tgcgcttgag ttagttctat
      601 acttggcagt ggaagtttgt cattatgagt tggtggaggt aaaaatccaa ccttgtttaa
      661 aaatgcaata tatataccaa cctcttctgg agtaacacct attccattag accaatcaac
      721 acctgtccat gcggtgtctt tctttaaaga tgccacggcg ttcctatcgt ctagttccgg
      781 agctttggta ctttcaggta agttgtctaa gaccatgtgt agcaaaggat acaacgcatt
      841 ttcttcattc ctgtcaataa cagacgcctc caatgatttc ttccatttag aatagctttc
      901 gatttcgaca tcgtaaccat aatcgtgtaa agtatacaag tagtctttga ataatattct
      961 tgggtgaccc gttacttgag caacggccaa ttcattttct ttgggaggat tcaaagacgt
     1021 agcaacaaca acacgagcaa catgatctac tggaaccata ttcacggaat tttcgatatc
     1081 tggaatctta cctaattgga ctgaaccttt caaaaatctc aataagaaat catctgtgtt
     1141 tgaagaacca ttggcagagg cacctgttac gtaacctggt ctgacaatac acccacgtag
     1201 gcccctttca cctgcacgtc taatgatgta ctcagcagcc catttggact gaccatatcc
     1261 accagtgagc ccgcttgcag agttcattaa atcgtctgat tctaaaatgc ctggcttccc
     1321 ttcgctaaca agtttatctg acaaattaaa gtagtattca gtgtcaagag tagaagtgga
     1381 ggaaacaaag tcaaagaact ttggcttgcc gacggcggct aagctcataa cattgatagt
     1441 tgaaataaca tttggatccc tcaatttggc atatggataa acccagtgaa ctaacgcacc
     1501 attatggata attatatcaa ctgtgtttgc caaatccatc cacttctcat ctgaaagacc
     1561 aaattggctt ttagataaat cgcctaatac aactttaata tttgaggcaa atttttcgtt
     1621 ccaagtacca taggtgatac ctgccttttg taatcttgca aatgcagctt cttcatcctt
     1681 ggccctgacg tgggcaaaca ctttgaaact gtagttcttt ggagaacgtc ctaacaaatc
     1741 tgcaaggatg taggagccca gaaatcctgt gacaccggta acaaacacat taattgttgt
     1801 ttttccttcg gcactattag gttcaacaaa atattctcga gaggggtacg aacttggtag
     1861 cgtctcaacc aatttcttgg cgtcttccgc ataatttgca gtgacatttt cgacgacctc
     1921 accttgagat gatccacccg atgatttaat tctgtcaatt tccgcggcaa aggcctttat
     1981 cgttggatac ttgaaaattg tgcccaatgg taaatcaact tgcagctttt tctttaaggt
     2041 aaaaatcatt ttggtagcca agatagaatg accacctaaa tcgaaaaacg aatcatctgg
     2101 tgatacagat gctggcttgg taggtaatat acttaaccat aagtctctaa cctcgcgctc
     2161 aacattggta aactgagagt cgtcagtttc agaaactgta ttttcagcta ccaaatttaa
     2221 ttgcttggga gttgggaatt gaagtttagg cttatcaact ttaccatttg gattcaatgg
     2281 tagtttatcc ataaccacaa tcaaggaagg catagcatag ctagccaatc ttttctttaa
     2341 gaaagtcctg atgtccttgg ataaaagatg gtaaccgatt aagcccttaa ctatagggtc
     2401 agtttcaacc tcctttggaa catcactttg gaacttagac aagtcatctg gcttgtcaaa
     2461 tcttgggacc ataaatgtga tcaatgttgg ctcattgtcg gcatttttgc gaactaaagt
     2521 aatgttttct cttaccaatg gatgttggga aatgtgcgta tctatttctc ctaattcgat
     2581 tctgaaccca cgaattttaa cttgatcatc agccctaccg caacattcac agtcaccgtt
     2641 tggtagataa cgacctaaat cacccgttct gtacaatcta tctcttggac ctaaccagaa
     2701 ttgtctccaa ggttcaccat tatccttatc caaataattc cagtgatctt tttcaacaaa
     2761 ccagttgttc acaaattttt ctttattcaa ttctggtaat cctctataac cttcggccaa
     2821 accacctgca cgaacataaa tctcacctat ttcgccaata ccacatattt gagtacgatc
     2881 gttcctgtta acaactagta gctgaacgtt caacatacct ttaccagcag gcatgacatc
     2941 tttcaatttt ttcaaaaagt ttggatcgtc attttttgat ttaacttcga aataagaaac
     3001 tgcacgctgt gtttcagtgg taccgtacat attaacaata cggcaatttt ctgccaaggt
     3061 ttgtaacctc agacaatcac gttttgttaa aatgtcaccc acaaagaacg catgatgtaa
     3121 cttagggaat ggtgtagtag cttgggcagt aagtaattga cccatggcag gtgttaaatg
     3181 ggtaactgtg caaccatact tactcatcca ttccgctaaa cggcccggtg taccaatatc
     3241 atcttgagta gggacataca attgggcacc taaaaataat ggtgtaaaca tatctctttg
     3301 aattggatca tgtgcaatac cgctcagcat tgtgaattta tcattttctg ttaagttgaa
     3361 ccttttggac atccaattga aataataagc caaggaaaaa tgtctaccaa gaacaccctt
     3421 aggaatacct tcggaaccag atgtgaaaga tagggttggg ttggaatctg gtccaactac
     3481 aacacctgtt ctggtgtctt tgtagtgatc atatggagcc aaaacatcct cgccattgtc
     3541 caatttgcca ccttcaatgg taccattttc ttgaatagcg atggaattga ttcttgaaac
     3601 aatctccaat tcatcattga tgtaatcttc tactagttga tccaattgtc cagcagctct
     3661 aataacaatc aacccacgtg gtttagcaac acctaagtaa atggtttgtc tggctggggg
     3721 atatgcaggg tcgataactg aaaaggttgc gccggctttc aagacaccca tcacacatac
     3781 catcaaatcc acacccctag aagaatagat catcactaca tcacctcttt tgatacctgt
     3841 tttaatcaaa taatgggcaa ctatgttaga agtgcggttg atgtcgcgat aagtgaaaga
     3901 acgggacttg tcggaattta gtgttggagt ctccacaaca caggttctct ctgggaaggc
     3961 ttcagcattg tcctggaaaa tgtcgtgaat acaccccacg aaatcgcacc agcccaagtt
     4021 cttagttgga tcaggtaaac tatccttgga tgatgcggtg atcagagaga ttttagttat
     4081 gcaattggat ggatcgctta gcgcagcagt caaatattga gtaaattggt ccgcaacaat
     4141 ggttactctt tcattcgaat acagtaagct gttataaatt aagttcaaaa catgcgcatt
     4201 attactggta tccaaattca aagcaaagtc cactaaatga tgcttgaact cgtctaattt
     4261 gaaatcttgg ttttccaaaa aggccaaacg gaacaactga ggggtccttt ccagatcttg
     4321 gcaactttga attttttcag ctagctcgtc aaaggaaaaa ttggcctcaa tagaattgag
     4381 cttgttcaac tcattgttaa ttgtagaata cagctcatta aatgaccacg ttggttgaat
     4441 attgaatctt aagattttgt tattcgcaat ataaagaaca atatcatcgt caccggttac
     4501 tctatatatc aatgcagccc atacactcaa agcgacagcg tatttgttag aaaaactatc
     4561 atgaggcaca tcgagctgag gtagctgtaa cgaatatgta gcttgtttcg tataaggttc
     4621 ttgttgtggg cgtaaaaagt catgtggtaa cactgaaaga gttggattat ccaacttctc
     4681 tatccagacc ttttcgttag tcattagtga gtaactctgt gatatctctc tataattagc
     4741 agtttttcac tgaaattcca ggaaaggtaa taaactcaga ttttttttta tactattggc
     4801 tgcttgttac ttatatatct tgaacttctc ccagcgggtc ttcaaataca tttgggcgat
     4861 gttcatgttc attaggcagg tatttcgaca ttgagtcaca cgcgaaaaac cgccggaatt
     4921 ttttatgtaa ttgcaagtgg aattgatcta tgcggtgtga aataccgcac agatgcgtaa
     4981 ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     5041 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     5101 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     5161 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     5221 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     5281 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     5341 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     5401 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat
     5461 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     5521 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     5581 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     5641 ggcgaattgg gtaccgggcc ccccctcgag gtcgacggta tcgataagct tgatatcgaa
     5701 ttcctgcagc ccgggggatc cactagttct agagcggccg ccaccgcggt ggagctccag
     5761 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     5821 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     5881 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     5941 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6001 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6061 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6121 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6181 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6241 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6301 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6361 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6421 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6481 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6541 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6601 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     6661 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6721 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6781 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6841 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6901 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6961 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7021 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7081 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7141 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7201 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7261 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7321 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7381 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7441 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7501 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7561 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7621 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7681 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7741 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7801 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7861 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7921 aataggggtt cagctgaagc ttcgacaacc cttaatataa cttcgtataa tgtatgctat
     7981 acgaagttat taggtctaga gatccccgcg cacatttccc cgaaaagtgc cacctgggtc
     8041 cttttcatca cgtactataa aaataattat aatttaaatt ttttaatata aatatataaa
     8101 ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg
     8161 taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc
     8221 aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg
     8281 tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt
     8341 ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt
     8401 ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca
     8461 aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac
     8521 gaggcgcgtg taagttacag gcaagcgatg cgagaaccct taatataact tcgtataatg
     8581 tatgctatac gaagttatta ggtgatatca gatccactag ctataaggtt gatattctca
     8641 cgagtaagcg gcacttgcta cttattgaca ttgcagattt ttggctacag aaatagtata
     8701 ttagagatta taattgctaa tcaaatcaaa atataaaatt agtaaaccaa accatttata
     8761 cccttcctta gtagttatgg attgtttttt aatgatattt ctgcaaacca aagaaagatt
     8821 gttatccaga tagaatttag ttttgatatt catttttttg ttgaagattg aacgccatat
     8881 ctgggcctca taattcaaaa gacggtgcca ttatcggtag cgtttcgcat tgtactggat
     8941 ggcgcgcctt cagaaatttc acagttgatg aatcgaaaag aatggtctca ttgcaacacg
     9001 taaggttaag atgtcccttt ttaccattat aggcaataaa tgaatcataa aacgaccgta
     9061 tactggtgaa atagtaggga gaacgagtac ctgtagtaaa aagtataaat catagttaat
     9121 cgggcaatgt ccctcgatca aggagtattg tgtcatgttc gagacaaacg ccaacatttt
     9181 tgtttctttt ggacaaatgt tgtttgcatt tatgatccgt tatattttga tctaatgtag
     9241 agttgcacgt agttcttact ggcaaagaaa tc
//
