LOCUS       sequence-104615-        5476 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Trp1 marker pXR vector uses for both plasmid assembly in vivo, as 
            well as targeted genomic integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Chou CC, Patel MT, Gartenberg MR
  TITLE     A series of conditional shuttle vectors for targeted genomic 
            integration in budding yeast.
  JOURNAL   FEMS Yeast Res. 2015 May;15(3):fov010. doi: 10.1093/femsyr/fov010. 
            Epub 2015 Mar 2.
  PUBMED    25736914
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2015-05"; volume: "15(3):fov010. doi"; pages: " 
            10.1093/femsyr/fov010. Epub 2015 Mar 2"
COMMENT     Sequence Label: pXRT1
FEATURES             Location/Qualifiers
     source          1..5476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     105..124
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        145..426
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             427..1101
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1204..1659)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1341..1362)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1553..1572
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(1636..2016)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     1785..1807
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1799..1816
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1800..1816
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1826..1845
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1826..1844
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1853..1960
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     1869..1885
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1870..1886
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1920..1936)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1920..1936)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1973..1993)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1973..1991)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2012..2028)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2012..2028)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2025..2047)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2036..2052
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2060..2066,2067..2084,2085..2090))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2105..2126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2243..2260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2414..3002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2494..2513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3173..3964,3965..4033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3796..3815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4034..4125)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    4160..4193
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4768..4801
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
       61 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
      121 tgtactgaga gtgcaccata gatcaacgac attactatat atataatata ggaagcattt
      181 aatagaacag catcgtaata tatgtgtact ttgcagttat gacgccagat ggcagtagtg
      241 gaagatattc tttattgaaa aatagcttgt caccttacgt acaatcttga tccggagctt
      301 ttcttttttt gccgattaag aattaattcg gtcgaaaaaa gaaaaggaga gggccaagag
      361 ggagggcatt ggtgactatt gagcacgtga gtatacgtga ttaagcacac aaaggcagct
      421 tggagtatgt ctgttattaa tttcacaggt agttctggtc cattggtgaa agtttgcggc
      481 ttgcagagca cagaggccgc agaatgtgct ctagattccg atgctgactt gctgggtatt
      541 atatgtgtgc ccaatagaaa gagaacaatt gacccggtta ttgcaaggaa aatttcaagt
      601 cttgtaaaag catataaaaa tagttcaggc actccgaaat acttggttgg cgtgtttcgt
      661 aatcaaccta aggaggatgt tttggctctg gtcaatgatt acggcattga tatcgtccaa
      721 ctgcatggag atgagtcgtg gcaagaatac caagagttcc tcggtttgcc agttattaaa
      781 agactcgtat ttccaaaaga ctgcaacata ctactcagtg cagcttcaca gaaacctcat
      841 tcgtttattc ccttgtttga ttcagaagca ggtgggacag gtgaactttt ggattggaac
      901 tcgatttctg actgggttgg aaggcaagag agccccgaaa gcttacattt tatgttagct
      961 ggtggactga cgccagaaaa tgttggtgat gcgcttagat taaatggcgt tattggtgtt
     1021 gatgtaagcg gaggtgtgga gacaaatggt gtaaaagact ctaacaaaat agcaaatttc
     1081 gtcaaaaatg ctaagaaata ggttattact gagtagtatt tatttaagta ttgtttgtgc
     1141 acttgccgat ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     1201 aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     1261 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     1321 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     1381 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     1441 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     1501 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     1561 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     1621 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg
     1681 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     1741 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     1801 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
     1861 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
     1921 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
     1981 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     2041 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     2101 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     2161 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     2221 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2281 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2341 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2401 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2461 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2521 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2581 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2641 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2701 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2761 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2821 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     2881 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2941 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3001 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3061 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3121 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     3181 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3241 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     3301 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     3361 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     3421 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     3481 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     3541 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     3601 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     3661 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     3721 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     3781 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3841 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3901 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3961 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     4021 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     4081 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttcagctga
     4141 agcttcgaca acccttaata taacttcgta taatgtatgc tatacgaagt tattaggtct
     4201 agagatcccc gcgcacattt ccccgaaaag tgccacctgg gtccttttca tcacgtacta
     4261 taaaaataat tataatttaa attttttaat ataaatatat aaattaaaaa tagaaagtaa
     4321 aaaaagaaat taaagaaaaa atagtttttg ttttccgaag atgtaaaaga ctctaggggg
     4381 atcgccaaca aatactacct tttatcttgc tcttcctgct ctcaggtatt aatgccgaat
     4441 tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc ctgtgatttt acattttact
     4501 tatcgttaat cgaatgtata tctatttaat ctgcttttct tgtctaataa atatatatgt
     4561 aaagtacgct ttttgttgaa attttttaaa cctttgttta tttttttttc ttcattccgt
     4621 aactcttcta ccttctttat ttactttcta aaatccaaat acaaaacata aaaataaata
     4681 aacacagagt aaattcccaa attattccat cattaaaaga tacgaggcgc gtgtaagtta
     4741 caggcaagcg atgcgagaac ccttaatata acttcgtata atgtatgcta tacgaagtta
     4801 ttaggtgata tcagatccac tagctataag gttgatattc tcacgagtaa gcggcacttg
     4861 ctacttattg acattgcaga tttttggcta cagaaatagt atattagaga ttataattgc
     4921 taatcaaatc aaaatataaa attagtaaac caaaccattt atacccttcc ttagtagtta
     4981 tggattgttt tttaatgata tttctgcaaa ccaaagaaag attgttatcc agatagaatt
     5041 tagttttgat attcattttt ttgttgaaga ttgaacgcca tatctgggcc tcataattca
     5101 aaagacggtg ccattatcgg tagcgtttcg cattgtactg gatggcgcgc cttcagaaat
     5161 ttcacagttg atgaatcgaa aagaatggtc tcattgcaac acgtaaggtt aagatgtccc
     5221 tttttaccat tataggcaat aaatgaatca taaaacgacc gtatactggt gaaatagtag
     5281 ggagaacgag tacctgtagt aaaaagtata aatcatagtt aatcgggcaa tgtccctcga
     5341 tcaaggagta ttgtgtcatg ttcgagacaa acgccaacat ttttgtttct tttggacaaa
     5401 tgttgtttgc atttatgatc cgttatattt tgatctaatg tagagttgca cgtagttctt
     5461 actggcaaag aaatct
//
