LOCUS       sequence-104616-        5570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ura3 marker pXR vector uses for both plasmid assembly in vivo, as 
            well as targeted genomic integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Chou CC, Patel MT, Gartenberg MR
  TITLE     A series of conditional shuttle vectors for targeted genomic 
            integration in budding yeast.
  JOURNAL   FEMS Yeast Res. 2015 May;15(3):fov010. doi: 10.1093/femsyr/fov010. 
            Epub 2015 Mar 2.
  PUBMED    25736914
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2015-05"; volume: "15(3):fov010. doi"; pages: " 
            10.1093/femsyr/fov010. Epub 2015 Mar 2"
COMMENT     Sequence Label: pXRU3
FEATURES             Location/Qualifiers
     source          1..5570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     105..124
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        148..363
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             364..1167
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1298..1753)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1435..1456)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1647..1666
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(1730..2110)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     1879..1901
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1893..1910
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1894..1910
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1920..1939
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1920..1938
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1947..2054
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     1963..1979
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1964..1980
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2014..2030)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2014..2030)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2067..2087)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2067..2085)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2106..2122)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2106..2122)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2119..2141)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2130..2146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2154..2160,2161..2178,2179..2184))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2199..2220
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2337..2354)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2508..3096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2588..2607)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3267..4058,4059..4127))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3890..3909
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4128..4219)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    4254..4287
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4862..4895
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
       61 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
      121 tgtactgaga gtgcaccata ccaccttttc aattcatcat ttttttttta ttcttttttt
      181 tgatttcggt ttccttgaaa tttttttgat tcggtaatct ccgaacagaa ggaagaacga
      241 aggaaggagc acagacttag attggtatat atacgcatat gtagtgttga agaaacatga
      301 aattgcccag tattcttaac ccaactgcac agaacaaaaa cctgcaggaa acgaagataa
      361 atcatgtcga aagctacata taaggaacgt gctgctactc atcctagtcc tgttgctgcc
      421 aagctattta atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt
      481 accaccaagg aattactgga gttagttgaa gcattaggtc ccaaaatttg tttactaaaa
      541 acacatgtgg atatcttgac tgatttttcc atggagggca cagttaagcc gctaaaggca
      601 ttatccgcca agtacaattt tttactcttc gaagacagaa aatttgctga cattggtaat
      661 acagtcaaat tgcagtactc tgcgggtgta tacagaatag cagaatgggc agacattacg
      721 aatgcacacg gtgtggtggg cccaggtatt gttagcggtt tgaagcaggc ggcagaagaa
      781 gtaacaaagg aacctagagg ccttttgatg ttagcagaat tgtcatgcaa gggctcccta
      841 tctactggag aatatactaa gggtactgtt gacattgcga agagcgacaa agattttgtt
      901 atcggcttta ttgctcaaag agacatgggt ggaagagatg aaggttacga ttggttgatt
      961 atgacacccg gtgtgggttt agatgacaag ggagacgcat tgggtcaaca gtatagaacc
     1021 gtggatgatg tggtctctac aggatctgac attattattg ttggaagagg actatttgca
     1081 aagggaaggg atgctaaggt agagggtgaa cgttacagaa aagcaggctg ggaagcatat
     1141 ttgagaagat gcggccagca aaactaaaaa actgtattat aagtaaatgc atgtatacta
     1201 aactcacaaa ttagagcttc aatttaatta tatcagttat taccctatgc ggtgtgaaat
     1261 accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg
     1321 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     1381 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     1441 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     1501 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     1561 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     1621 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     1681 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     1741 ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     1801 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     1861 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt
     1921 aatacgactc actatagggc gaattgggta ccgggccccc cctcgaggtc gacggtatcg
     1981 ataagcttga tatcgaattc ctgcagcccg ggggatccac tagttctaga gcggccgcca
     2041 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     2101 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     2161 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     2221 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     2281 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2341 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2401 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2461 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2521 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2581 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2641 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2701 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2761 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2821 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2881 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2941 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3001 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3061 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3121 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3181 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3241 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3301 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3361 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3421 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3481 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3541 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3601 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3661 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3721 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3781 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3841 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3901 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3961 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4021 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4081 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4141 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4201 atttagaaaa ataaacaaat aggggttcag ctgaagcttc gacaaccctt aatataactt
     4261 cgtataatgt atgctatacg aagttattag gtctagagat ccccgcgcac atttccccga
     4321 aaagtgccac ctgggtcctt ttcatcacgt actataaaaa taattataat ttaaattttt
     4381 taatataaat atataaatta aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt
     4441 tttgttttcc gaagatgtaa aagactctag ggggatcgcc aacaaatact accttttatc
     4501 ttgctcttcc tgctctcagg tattaatgcc gaattgtttc atcttgtctg tgtagaagac
     4561 cacacacgaa aatcctgtga ttttacattt tacttatcgt taatcgaatg tatatctatt
     4621 taatctgctt ttcttgtcta ataaatatat atgtaaagta cgctttttgt tgaaattttt
     4681 taaacctttg tttatttttt tttcttcatt ccgtaactct tctaccttct ttatttactt
     4741 tctaaaatcc aaatacaaaa cataaaaata aataaacaca gagtaaattc ccaaattatt
     4801 ccatcattaa aagatacgag gcgcgtgtaa gttacaggca agcgatgcga gaacccttaa
     4861 tataacttcg tataatgtat gctatacgaa gttattaggt gatatcagat ccactagcta
     4921 taaggttgat attctcacga gtaagcggca cttgctactt attgacattg cagatttttg
     4981 gctacagaaa tagtatatta gagattataa ttgctaatca aatcaaaata taaaattagt
     5041 aaaccaaacc atttataccc ttccttagta gttatggatt gttttttaat gatatttctg
     5101 caaaccaaag aaagattgtt atccagatag aatttagttt tgatattcat ttttttgttg
     5161 aagattgaac gccatatctg ggcctcataa ttcaaaagac ggtgccatta tcggtagcgt
     5221 ttcgcattgt actggatggc gcgccttcag aaatttcaca gttgatgaat cgaaaagaat
     5281 ggtctcattg caacacgtaa ggttaagatg tcccttttta ccattatagg caataaatga
     5341 atcataaaac gaccgtatac tggtgaaata gtagggagaa cgagtacctg tagtaaaaag
     5401 tataaatcat agttaatcgg gcaatgtccc tcgatcaagg agtattgtgt catgttcgag
     5461 acaaacgcca acatttttgt ttcttttgga caaatgttgt ttgcatttat gatccgttat
     5521 attttgatct aatgtagagt tgcacgtagt tcttactggc aaagaaatct
//
