LOCUS       sequence-104619-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty Backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_chaining vector
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        269..373
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(592..611)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
       61 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      121 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      181 cgcagccgaa cgaccgagcg cagcgagtca gtgagccatg atatcaaacg cgtaacctcg
      241 agaacgtcga ccaattcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      301 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      361 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      421 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      481 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
      541 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      601 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
      661 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
      721 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
      781 tctgacaacg atcggaggac cgaaggagct aaccgc
//
