LOCUS       sequence-104647-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with mAzamiGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_mAzamiGreen
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..687)
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQA"
     CDS             complement(13..687)
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQA"
ORIGIN
        1 gaattggtcg acggcctggc tgggcagcat ggagtacctg gccacggcgt tctcgtacag
       61 cttcaccttg ttgtagtcct tgtcgtgctt caggatctcg atcctgtggt cgatcttgtg
      121 ggcgtcgggc agcctcacct ccttcttggc cttgtaggtg gtcttgaagt cgcacctgta
      181 gtggccgccg ccctccagca gcagcctcat gttcacgtcg cccttcagca cgccgtcctc
      241 cacgtacatc ttctcggtgc tgggctccca cttcagggtc ttcttctgca tcacggggcc
      301 gttggggggg aagttggtgc cgtcgaacct gatgtcgtag aagaagcagt cgcccctcat
      361 gctgatgttg ctggtggcgg tgcagatgcc ctggtcctcg taggtcatgc tcctctccca
      421 gtggtagccc tcggggaagg tctgcttgaa gtagtcctgg atgtcggcgg ggtacttggt
      481 gaaggccctg ttgccgtact ggaacacggt ggtcaggatg tcgtaggcga agggcagggg
      541 ggcgccctcg gtcacgttca ggtccaggat ctgggtgccc tcgtaggggt tgcccttgcc
      601 ctcgccctcg atcacgaagt tgtggccgtt cacggtgccc ctcatgcaca gcttgatctt
      661 catctcgggc ttgatcacgc tcaccatctc gagttacgcg tttgatatca tggctcactg
      721 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactc
//
