LOCUS       sequence-104656-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_tdTomato
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(96..119)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(294..313)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     563..581
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gaattggtcg accttgtaca gctcgtccat gccgtacagg aacaggtggt ggcggccctc
       61 ggagcgctcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt
      121 ggtgtccacg tagtagtagc cgggcagttg cacgggcttc ttggccatgt agatggtctt
      181 gaactccacc aggtagtggc cgccgtcctt cagcttcagg gcctggtgga tctcgccctt
      241 cagcacgccg tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tggtcttctt
      301 ctgcattacg gggccgtcgg gggggaagtt ggtgccgcgc atcttcacct tgtagatcag
      361 cgtgccgtcc tgcagggagg agtcctgggt cacggtcacc agaccgccgt cctcgaagtt
      421 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcttgtaat cggggatgtc
      481 ggcggggtgc ttcacgtacg ccttggagcc gtacatgaac tggggggaca ggatgtccca
      541 ggcgaagggc agggggccgc ccttggtcac cttcagcttg gcggtctggg tgccctcgta
      601 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcatgg agccctccat
      661 gcgcaccttg aagcgcatga actctttgat gacggccatg ttgttg
//
