LOCUS       sequence-104663-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with E2-Crimson.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_E2-Crimson
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..687)
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(270..289)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gaattggtcg acctggaaca ggtggtggcg ggcctcggcg cgctcgtact gctccaccac
       61 ggtgtagtcc tcgttgtggg aggtgatgtc gagcttgtag tccacgtagt ggtagccggg
      121 cagcttcacg ggcttcttgg ccatgtagat ggacttgaac tcacacaggt agtggccgcc
      181 gcccttcagc ttcagcgcca tgtggttctc gcccttcagc acgccgtcgc gggggtagtt
      241 gcgctcagtg gagggctccc agcccagagt cttcttctgc attacggggc cgtcggaggg
      301 gaagttcacg ccgatgaact tcacgtggta gatgagggtg ccgtcctgca gggaggagtc
      361 ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc acttgaagcc
      421 ctcggggaag gactgcttga ggtagtcggg gatgtcggcg gggtgcttga tgtacgcctt
      481 ggagccgtag aagaactggg gggacaggat gtcccaggcg aagggcaggg ggccgccctt
      541 ggtcacttgc agcttggcgg tctgggtgcc ctcgtagggc ttgccctcgc ccacgccctc
      601 gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc accttgaagc gcatgaaggg
      661 cttgatgacg ttctcagtgc tatccatctc gagttacgcg tttgatatca tggctcactg
      721 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactc
//
