LOCUS       sequence-104676-         633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with PGK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_PGK
FEATURES             Location/Qualifiers
     source          1..633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(15..514)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(63..82)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     456..479
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gaattggtcg acgtcgaaag gcccggagat gaggaagagg agaacagcgc ggcagacgtg
       61 cgcttttgaa gcgtgcagaa tgccgggcct ccggaggacc ttcgggcgcc cgccccgccc
      121 ctgagcccgc ccctgagccc gcccccggac ccaccccttc ccagcctctg agcccagaaa
      181 gcgaaggagc aaagctgcta ttggccgctg ccccaaaggc ctacccgctt ccattgctca
      241 gcggtgctgt ccatctgcac gagactagtg agacgtgcta cttccatttg tcacgtcctg
      301 cacgacgcga gctgcggggc gggggggaac ttcctgacta ggggaggagt agaaggtggc
      361 gcgaaggggc caccaaagaa cggagccggt tggcgcctac cggtggatgt ggaatgtgtg
      421 cgaggccaga ggccacttgt gtagcgccaa gtgcccagcg gggctgctaa agcgcatgct
      481 ccagactgcc ttgggaaaag cgcctcccct acccggtaga ctcgagttac gcgtttgata
      541 tcatggctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      601 tcaaaggcgg taatacggtt atccacagaa tca
//
