LOCUS       sequence-104678-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with minPGK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_minPGK
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     364..383
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        457..561
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
       61 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
      121 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
      181 cgagcgcagc gagtcagtga gccatgatat caaacgcgta actcgagctt tggggcagcg
      241 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
      301 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
      361 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
      421 cgggcctttc gacgtcgacc aattcgggga aatgtgcgcg gaacccctat ttgtttattt
      481 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      541 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
      601 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      661 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagc
//
