LOCUS       sequence-104679-         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with minPGK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_minPGK
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(77..96)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(506..525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgcacattt ccccgaattg gtcgacgtcg aaaggcccgg agatgaggaa gaggagaaca
       61 gcgcggcaga cgtgcgcttt tgaagcgtgc agaatgccgg gcctccggag gaccttcggg
      121 cgcccgcccc gcccctgagc ccgcccctga gcccgccccc ggacccaccc cttcccagcc
      181 tctgagccca gaaagcgaag gagcaaagct gctattggcc gctgccccaa agctcgagtt
      241 acgcgtttga tatcatggct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      301 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      361 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      421 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      481 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      541 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      601 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      661 cgctccaagc tgggctgtgt gcacg
//
