LOCUS       sequence-104700-         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with Tet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_Tet
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(291..315)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    join(342..360,361..383,384..402,403..425,426..444,445..467,
                     468..486,487..509,510..528,529..551,552..570,571..593,
                     594..612)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
       61 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
      121 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
      181 cgagcgcagc gagtcagtga gccatggtcg acgaattcgg ggccgcggag gctggatcgg
      241 tcccggtgtc ttctatggag gtcaaaacag cgtggatggc gtctccaggc gatctgacgg
      301 ttcactaaac gagctctgct tatataggtc gagtttacca ctccctatca gtgatagaga
      361 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
      421 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
      481 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
      541 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
      601 cagtgataga gaaaagtgaa agtcgagctc ggtaccctcg agttacgcgt aatt
//
