LOCUS       sequence-104701-         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with Tet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_Tet
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(44..62,63..85,86..104,105..127,128..146,147..169,
                     170..188,189..211,212..230,231..253,254..272,273..295,
                     296..314)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     341..365
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gaattacgcg taactcgagg gtaccgagct cgactttcac ttttctctat cactgatagg
       61 gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta aactcgactt
      121 tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact
      181 gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact
      241 cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct
      301 atcactgata gggagtggta aactcgacct atataagcag agctcgttta gtgaaccgtc
      361 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      421 ccagcctccg cggccccgaa ttcgtcgacc atggctcact gactcgctgc gctcggtcgt
      481 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      541 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      601 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      661 tcgacgctca agtcagaggt ggcg
//
