LOCUS       sequence-104702-         620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with BiTet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_BiTet
FEATURES             Location/Qualifiers
     source          1..620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(238..262)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    join(333..351,352..374,375..393,394..416,417..435,436..458,
                     459..477,478..500,501..519,520..542,543..561,562..584,
                     585..603)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
       61 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
      121 tgtatataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
      181 cgagcgcagc gagtcagtga gccatggtcg accagctgag atctgaattc tccaggcgat
      241 ctgacggttc actaaacgag ctctgcttat ataggcctcc caccgtacac gccacctcga
      301 catactcgac cagcgagctc gactttcact tttctctatc actgataggg agtggtaaac
      361 tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc
      421 tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg
      481 gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact
      541 tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag
      601 ggagtggtaa actcgaccta
//
