LOCUS       sequence-104712-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 400 / 504, Emission: 515 / 
            517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   York AG, Ghitani A, Vaziri A, Davidson MW, Shroff H
  TITLE     Confined activation and subdiffractive localization enables 
            whole-cell PALM with genetically expressed probes.
  JOURNAL   Nat Methods. 2011 Apr;8(4):327-33. doi: 10.1038/nmeth.1571. Epub 
            2011 Feb 13.
  PUBMED    21317909
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1571"; journalName: "Nat Methods"; date: "2011-04"; 
            volume: "8"; issue: "4"; pages: "327-33"
COMMENT     Sequence Label: PA-mCherry1-Mito-7
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..97
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      125..134
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             131..841
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(276..294)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcatgtccgt cctgacgccg ctgctgctgc ggggcttgac
       61 aggctcggcc cggcggctcc cagtgccgcg cgccaagatc cattcgttgg gggatccacc
      121 ggtcgccacc atggtgagca agggcgagga ggataacatg gccatcatta aggagttcat
      181 gcgcttcaag gtgcacatgg aggggtccgt gaacggccac gtgttcgaga tcgagggcga
      241 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
      301 ccccctgccc ttcacctggg acatcctgag ccctcagttc atgtacggct ccaatgccta
      361 cgtgaagcac cccgccgaca tccccgacta ctttaagctg tccttccccg agggcttcaa
      421 gtgggagcgc gtgatgaaat tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
      481 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
      541 cggccccgtg atgcagaaga agaccatggg ctgggaggcc ctctccgagc ggatgtaccc
      601 cgaggacggc gccctgaagg gcgaggtcaa gcccagagtg aagctgaagg acggcggcca
      661 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
      721 ctacaacgtc aaccgcaagc tggacatcac cagccacaac gaggactaca ccatcgtgga
      781 gcagtacgag agagccgagg gccgccactc caccggcggc atggacgagc tgtacaagta
      841 agcggccgcg actctagatc ataatcagcc ataccacat
//
