LOCUS       sequence-104718-         500 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for F-actin LifeAct-mCherry expressed under 
            AtUBQ10 promoter .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMU-ACTLr
FEATURES             Location/Qualifiers
     source          1..500
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..84)
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     protein_bind    complement(87..111)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggccatgtta tcctcctcgc ccttgctcac catttcttcc tttgagatgc tttcgaattt
       61 cttgatcaaa tctgcgacac ccatgaagcc tgcttttttg tacaaacttg cctgttaatc
      121 agaaaaactc agattaatcg acaaattcga tcgcacaaac tagaaactaa caccagatct
      181 agatagaaat cacaaatcga agagtaatta ttcgacaaaa ctcaaattat ttgaacaaat
      241 cggatgatat ctatgaaacc ctaatcgaga attaagatga tatctaacga tcaaacccag
      301 aaaatcgtct tcnatctaag attaacagaa tctaaaccaa agaacatata cgaaattggg
      361 atcgaacgaa aacaaaatcg aagattttga gagaataagg aacacagaaa tttaccttga
      421 tcacggtaga gagaattgag agaaagtttt taagattttg agaaattgaa atctgaattg
      481 tgaagaagaa gagctctttg
//
