LOCUS       sequence-104723-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses beta actin with both a PAGFP and mRFP tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Kueh HY, Charras GT, Mitchison TJ, Brieher WM
  TITLE     Actin disassembly by cofilin, coronin, and Aip1 occurs in bursts and
            is inhibited by barbed-end cappers.
  JOURNAL   J Cell Biol. 2008 Jul 28;182(2):341-53. doi: 10.1083/jcb.200801027.
  PUBMED    18663144
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2008-07-28- 28"; volume: "182"; 
            issue: "2"; pages: "341-53"
COMMENT     Sequence Label: Actin-mRFP-PAGFP
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..719
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             45..719
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(175..193)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     443..462
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     637..660
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(786..807)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcatggcc tcctccgagg
       61 acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg aacggccacg
      121 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      181 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc cctcagttcc
      241 agtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
      301 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
      361 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
      421 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
      481 ccaccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag atgaggctga
      541 agctgaagga cggcggccac tacgacgccg aggtcaagac cacctacatg gccaagaagc
      601 ccgtgcagct gcccggcgcc tacaagaccg acatcaagct ggacatcacc tcccacaacg
      661 aggactacac catcgtggaa cagtacgagc gcgccgaggg ccgccactcc accggcgcct
      721 ccggactcag atctcgaggc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      781 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      841 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      901 tgcccgtgcc ctggcccacc ctcgtgacca ccttcagcta cggcgtgcag tgcttcagcc
      961 gctaccccga ccacatg
//
