LOCUS       sequence-104727-         638 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with rTA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_tTA2
FEATURES             Location/Qualifiers
     source          1..638
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..132)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
ORIGIN
        1 gaattggtcg actcatccgg ggagcatgtc aaggtcaaaa tcgtcaagag cgtcagcagg
       61 cagcatatca aggtcaaagt cgtcaagggc atcggctggg agcatgtcta agtcaaaatc
      121 gtcaagggcg tcggccggcc cgccgctttc gcactttagc tgtttctcca ggccacatat
      181 gattagttcc aggccgaaaa ggaaggcagg ttcggctccc tgatggtcga acagctcaat
      241 tgcttgtctc agaagtgggg gcatagaatc ggtggtaggt gtctctcttt cctcttttgc
      301 tacttgatgc tcctgatcct ccaatacgca gcccagtgta aagtggccca cggcggacag
      361 agcgtacagt gcgttctcca gggagaagcc ttgctgacac aggaacgcga gctgattttc
      421 cagggtttcg tactgtttct ctgttgggcg ggtgccgaga tgcactttag ccccgtcgcg
      481 atgtgagagg agagcacagc ggaatgactt ggcgttgttc cgcagaaagt cttgccatga
      541 ctcgccttcc agggggcaga agtgggtatg atgcctgtcc agcatctcga ttgccagggc
      601 atcgagcagg gcccgcttgt tcttcacgtg ccagtaca
//
