LOCUS       sequence-104730-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with loxP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_loxP
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    228..261
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        287..391
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(610..629)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
       61 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
      121 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
      181 cgagcgcagc gagtcagtga gccatgatat caaacgcgta actcgagata acttcgtata
      241 atgtatgcta tacgaagtta tgcgtcgacc aattcgggga aatgtgcgcg gaacccctat
      301 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      361 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      421 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      481 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      541 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      601 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      661 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      721 tcttacggat ggcatgacag taagagaatt atgcagtgct g
//
