LOCUS       sequence-104743-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with PEST2D .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_PEST2D
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        380..484
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(703..722)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
       61 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
      121 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
      181 cgagcgcagc gagtcagtga gccatgatat caaacgcgta actcgagaaa cttagccatg
      241 gcttcccgcc ggaggtggag gagcaggatg atggcacgct gcccatgtct tgtgcccagg
      301 agagcgggat ggaccgtcac cctgccgcct gtgcttctgc taggatcaat gtgtaggtcg
      361 accaattcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      421 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      481 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      541 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      601 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      661 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      721 at
//
