LOCUS       sequence-104754-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with PGK::HygroR-bGHpA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_PGK::HygroR-bGHpA
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    12..236
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(355..374)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gaattggtcg accatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc
       61 ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca
      121 atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt
      181 caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag gcacagctcg
      241 acctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc
      301 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc
      361 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat
      421 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt
      481 cacgacgaga tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg
      541 cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg
      601 agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc
      661 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
      721 gtgagatgac aggagatcct g
//
