LOCUS       sequence-104755-        1128 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of N-terminal fusion protein of CNA35 with 
            m-Turquoise-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Aper SJ, van Spreeuwel AC, van Turnhout MC, van der Linden AJ, 
            Pieters PA, van der Zon NL, de la Rambelje SL, Bouten CV, Merkx M
  TITLE     Colorful Protein-Based Fluorescent Probes for Collagen Imaging.
  JOURNAL   PLoS One. 2014 Dec 9;9(12):e114983. doi: 
            10.1371/journal.pone.0114983. eCollection 2014.
  PUBMED    25490719
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-12-9"; volume: "9(12):e114983. doi"; pages: " 
            10.1371/journal.pone.0114983. eCollection 2014"
COMMENT     Sequence Label: pET28a-mTurquoise2-CNA35
FEATURES             Location/Qualifiers
     source          1..1128
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             124..375
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     312..333
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
       61 ggcaacatcc tggggcacaa gctggagtac aactacttta gcgacaacgt ctatatcacc
      121 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac
      181 ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      241 ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag
      301 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      361 gacgagctgt acaaggaatt ccacggatcc gcacgagata tttcatcaac gaatgttaca
      421 gatttaactg tatcaccgtc taagatagaa gatggtggta aaacgacagt aaaaatgacg
      481 ttcgacgata aaaatggaaa aatacaaaat ggtgacatga ttaaagtggc atggccgaca
      541 agcggtacag taaagataga gggttatagt aaaacagtac cattaactgt taaaggtgaa
      601 caggtgggtc aagcagttat tacaccagac ggtgcaacaa ttacattcaa tgataaagta
      661 gaaaaattaa gtgatgtttc gggatttgca gaatttgaag tacaaggaag aaatttaacg
      721 caaacaaata cttcagatga caaagtagct acgataacat ctgggaataa atcaacgaat
      781 gttacggttc ataaaagtga agcgggaaca agtagtgttt tctattataa aacgggagat
      841 atgctaccag aagatacgac acatgtacga tggtttttaa atattaacaa tgaaaaaagt
      901 tatgtatcga aagatattac tataaaggat cagattcaag gtggacagca gttagattta
      961 agcacattaa acattaatgt gacaggtaca catagcaatt attatagtgg acaaagtgca
     1021 attactgatt ttgaaaaagc ctttccaggt tctaaaataa ctgttgataa tacgaagaac
     1081 acaattgatg taacaattcc acaaggctat gggtcatata atagtttt
//
