LOCUS       sequence-104765-        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of N-terminal fusion protein of CNA35 with 
            mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Aper SJ, van Spreeuwel AC, van Turnhout MC, van der Linden AJ, 
            Pieters PA, van der Zon NL, de la Rambelje SL, Bouten CV, Merkx M
  TITLE     Colorful Protein-Based Fluorescent Probes for Collagen Imaging.
  JOURNAL   PLoS One. 2014 Dec 9;9(12):e114983. doi: 
            10.1371/journal.pone.0114983. eCollection 2014.
  PUBMED    25490719
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-12-9"; volume: "9(12):e114983. doi"; pages: " 
            10.1371/journal.pone.0114983. eCollection 2014"
COMMENT     Sequence Label: pET28a-mCherry-CNA35
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     67..86
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        67..85
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    86..110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             125..147
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             166..183
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             193..210
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg
       61 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa
      121 taattttgtt taactttaag aaggagatat accatgggca gcagccatca tcatcatcat
      181 cacagcagcg gcctggtgcc gcgcggcagc catatggcta gcatggtttc taagggcgaa
      241 gaggacaata tggctatcat caaagagttc atgcgtttta aagtacacat ggaaggctcc
      301 gttaacggtc acgagtttga aattgaaggt gagggcgaag gtcgcccgta cgaaggcact
      361 caaacggcga agctgaaagt gaccaaaggt ggcccactgc cgttcgcttg ggacattctg
      421 tccccgcaat tcatgtatgg ttctaaagcg tacgtgaaac acccggcgga tattccggat
      481 tacctgaaac tgtctttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac
      541 ggtggtgttg taacggttac tcaggactct agcctgcagg acggcgaatt tatctataaa
      601 gtaaaactgc gtggtactaa cttcccgtct gatggcccgg ttatgcaaaa gaaaactatg
      661 ggttgggaag cttctagcga acgcatgtac ccggaggacg gtgccctgaa aggtgaaatc
      721 aaacaacgcc tgaaactgaa ggacggcggc cactacgatg cggaagtgaa aaccacgtac
      781 aaagcgaaga aaccagtaca gctgcctggt gcctacaatg tgaatatcaa actggatatt
      841 acctcccata acgaagacta taccatcgtc gaacagtatg aacgtgctga aggtcgccat
      901 agcaccggtg gcatggatga gttatacaag gaattccacg gatccgcacg agatatttca
      961 tcaacgaatg ttacagattt aactgtatca ccgtctaaga tagaagatgg tggtaaaacg
     1021 acagtaaaaa tgacgttcga cgataaaaat ggaaaaatac aaaatggtga catgattaaa
     1081 gtggcatggc cgacaagcgg tacagtaa
//
