LOCUS       sequence-104773-         606 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of N-terminal fusion protein of CNA35 with 
            tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Aper SJ, van Spreeuwel AC, van Turnhout MC, van der Linden AJ, 
            Pieters PA, van der Zon NL, de la Rambelje SL, Bouten CV, Merkx M
  TITLE     Colorful Protein-Based Fluorescent Probes for Collagen Imaging.
  JOURNAL   PLoS One. 2014 Dec 9;9(12):e114983. doi: 
            10.1371/journal.pone.0114983. eCollection 2014.
  PUBMED    25490719
REFERENCE   2  (bases 1 to 606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-12-9"; volume: "9(12):e114983. doi"; pages: " 
            10.1371/journal.pone.0114983. eCollection 2014"
COMMENT     Sequence Label: pET28a-tdTomato-CNA35
FEATURES             Location/Qualifiers
     source          1..606
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(102..125)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(300..319)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     569..587
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ggtgccggag ctgccgctgc cggtgctgcc ggtgccatgc cccaggaaca ggtggtggcg
       61 gccctcggag cgctcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc
      121 cagcttggtg tccacgtagt agtagccggg cagttgcacg ggcttcttgg ccatgtagat
      181 ggtcttgaac tccaccaggt agtggccgcc gtccttcagc ttcagggcct ggtggatctc
      241 gcccttcagc acgccgtcgc gggggtacag gcgctcggtg gaggcctccc agcccatggt
      301 cttcttctgc attacggggc cgtcgggggg gaagttggtg ccgcgcatct tcaccttgta
      361 gatcagcgtg ccgtcctgca gggaggagtc ctgggtcacg gtcaccagac cgccgtcctc
      421 gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttct tgtaatcggg
      481 gatgtcggcg gggtgcttca cgtacgcctt ggagccgtac atgaactggg gggacaggat
      541 gtcccaggcg aagggcaggg ggccgccctt ggtcaccttc agcttggcgg tctgggtgcc
      601 ctcgta
//
