LOCUS       sequence-104778-        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of N-terminal fusion protein of CNA35 with 
            tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Aper SJ, van Spreeuwel AC, van Turnhout MC, van der Linden AJ, 
            Pieters PA, van der Zon NL, de la Rambelje SL, Bouten CV, Merkx M
  TITLE     Colorful Protein-Based Fluorescent Probes for Collagen Imaging.
  JOURNAL   PLoS One. 2014 Dec 9;9(12):e114983. doi: 
            10.1371/journal.pone.0114983. eCollection 2014.
  PUBMED    25490719
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-12-9"; volume: "9(12):e114983. doi"; pages: " 
            10.1371/journal.pone.0114983. eCollection 2014"
COMMENT     Sequence Label: pET28a-tdTomato-CNA35
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             980..997
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1050..1068)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ggatccgcac gagatatttc atcaacgaat gttacagatt taactgtatc accgtctaag
       61 atagaagatg gtggtaaaac gacagtaaaa atgacgttcg acgataaaaa tggaaaaata
      121 caaaatggtg acatgattaa agtggcatgg ccgacaagcg gtacagtaaa gatagagggt
      181 tatagtaaaa cagtaccatt aactgttaaa ggtgaacagg tgggtcaagc agttattaca
      241 ccagacggtg caacaattac attcaatgat aaagtagaaa aattaagtga tgtttcggga
      301 tttgcagaat ttgaagtaca aggaagaaat ttaacgcaaa caaatacttc agatgacaaa
      361 gtagctacga taacatctgg gaataaatca acgaatgtta cggttcataa aagtgaagcg
      421 ggaacaagta gtgttttcta ttataaaacg ggagatatgc taccagaaga tacgacacat
      481 gtacgatggt ttttaaatat taacaatgaa aaaagttatg tatcgaaaga tattactata
      541 aaggatcaga ttcaaggtgg acagcagtta gatttaagca cattaaacat taatgtgaca
      601 ggtacacata gcaattatta tagtggacaa agtgcaatta ctgattttga aaaagccttt
      661 ccaggttcta aaataactgt tgataatacg aagaacacaa ttgatgtaac aattccacaa
      721 ggctatgggt catataatag tttttcaatt aactacaaaa ccaaaattac gaatgaacag
      781 caaaaagagt ttgttaataa ttcacaagct tggtatcaag agcatggtaa ggaagaagtg
      841 aacgggaaat catttaatca tactgtgcac aatattaatg ctaatgccgg tattgaaggt
      901 actgtaaaag gtgaattaaa agttttaaaa caggataaag ataccaaggc ttcagtcgac
      961 ctgtaaggca ttgctcgagc accaccacca ccaccactga gatccggctg ctaacaaagc
     1021 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     1081 ggcctctaa
//
