LOCUS       sequence-104780-        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of N-terminal fusion protein of CNA35 with 
            tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Aper SJ, van Spreeuwel AC, van Turnhout MC, van der Linden AJ, 
            Pieters PA, van der Zon NL, de la Rambelje SL, Bouten CV, Merkx M
  TITLE     Colorful Protein-Based Fluorescent Probes for Collagen Imaging.
  JOURNAL   PLoS One. 2014 Dec 9;9(12):e114983. doi: 
            10.1371/journal.pone.0114983. eCollection 2014.
  PUBMED    25490719
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-12-9"; volume: "9(12):e114983. doi"; pages: " 
            10.1371/journal.pone.0114983. eCollection 2014"
COMMENT     Sequence Label: pET28a-tdTomato-CNA35
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(107..154)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     150..168
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(221..238)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
       61 cagggcgcgt cccattcgcc aatccggata tagttcctcc tttcagcaaa aaacccctca
      121 agacccgttt agaggcccca aggggttatg ctagttattg ctcagcggtg gcagcagcca
      181 actcagcttc ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct
      241 cgagcaatgc cttacaggtc gactgaagcc ttggtatctt tatcctgttt taaaactttt
      301 aattcacctt ttacagtacc ttcaataccg gcattagcat taatattgtg cacagtatga
      361 ttaaatgatt tcccgttcac ttcttcctta ccatgctctt gataccaagc ttgtgaatta
      421 ttaacaaact ctttttgctg ttcattcgta attttggttt tgtagttaat tgaaaaacta
      481 ttatatgacc catagccttg tggaattgtt acatcaattg tgttcttcgt attatcaaca
      541 gttattttag aacctggaaa ggctttttca aaatcagtaa ttgcactttg tccactataa
      601 taattgctat gtgtacctgt cacattaatg tttaatgtgc ttaaatctaa ctgctgtcca
      661 ccttgaatct gatcctttat agtaatatct ttcgatacat aacttttttc attgttaata
      721 tttaaaaacc atcgtacatg tgtcgtatct tctggtagca tatctcccgt tttataatag
      781 aaaacactac ttgttcccgc ttcactttta tgaaccgtaa cattcgttga tttattccca
      841 gatgttatcg tagctacttt gtcatctgaa gtatttgttt gcgttaaatt tcttccttgt
      901 acttcaaatt ctgcaaatcc cgaaacatca cttaattttt ctactttatc attgaatgta
      961 attgttgcac cgtctggtgt aataactgct tgacccacct gttcaccttt aacagttaat
     1021 ggtactgttt tactataacc ctctatcttt actgtaccgc ttgtcggcca tgccacttta
     1081 atcatgtcac cattt
//
