LOCUS       sequence-104782-         628 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with PGK::ZeoR-bGHpA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_PGK::ZeoR-bGHpA
FEATURES             Location/Qualifiers
     source          1..628
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    5..229
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(236..610)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcgaccatag agcccaccgc atccccagca tgcctgctat tgtcttccca atcctccccc
       61 ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag acaatgcgat
      121 gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa
      181 ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagc tcgacttagt
      241 cctgctcctc ggccacgaag tgcacgcagt tgccggccgg gtcgcgcagg gcgaactccc
      301 gcccccacgg ctgctcgccg atctcggtca tggccggccc ggaggcgtcc cggaagttcg
      361 tggacacgac ctccgaccac tcggcgtaca gctcgtccag gccgcgcacc cacacccagg
      421 ccagggtgtt gtccggcacc acctggtcct ggaccgcgct gatgaacagg gtcacgtcgt
      481 cccggaccac accggcgaag tcgtcctcca cgaagtcccg ggagaacccg agccggtcgg
      541 tccagaactc gaccgctccg gcgacgtcgc gcgcggtgag caccggaacg gcactggtca
      601 acttggccat ggtggcgact cgacgtcg
//
