LOCUS       sequence-104787-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of N-terminal fusion protein of CNA35 with 
            EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Aper SJ, van Spreeuwel AC, van Turnhout MC, van der Linden AJ, 
            Pieters PA, van der Zon NL, de la Rambelje SL, Bouten CV, Merkx M
  TITLE     Colorful Protein-Based Fluorescent Probes for Collagen Imaging.
  JOURNAL   PLoS One. 2014 Dec 9;9(12):e114983. doi: 
            10.1371/journal.pone.0114983. eCollection 2014.
  PUBMED    25490719
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-12-9"; volume: "9(12):e114983. doi"; pages: " 
            10.1371/journal.pone.0114983. eCollection 2014"
COMMENT     Sequence Label: pET28a-EGFP-CNA35
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     314..335
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
       61 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
      121 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
      181 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      241 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
      301 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      361 tggacgagct gtacaagtcc ggagaattcc acggatccgc acgagatatt tcatcaacga
      421 atgttacaga tttaactgta tcaccgtcta agatagaaga tggtggtaaa acgacagtaa
      481 aaatgacgtt cgacgataaa aatggaaaaa tacaaaatgg tgacatgatt aaagtggcat
      541 ggccgacaag cggtacagta aagatagagg gttatagtaa aacagtacca ttaactgtta
      601 aaggtgaaca ggtgggtcaa gcagttatta caccagacgg tgcaacaatt acattcaatg
      661 ataaagtaga aaaattaagt gatgtttcgg gatttgcaga atttgaagta caaggaagaa
      721 atttaacgca aacaaatact tcagatgaca aagtagctac gataacatct gggaataaat
      781 caacgaatgt tacggttcat aaaagtgaag cgggaacaag tagtgttttc tattataaaa
      841 cgggagatat gctaccagaa gatacgacac atgtacgatg gtttttaaat attaacaatg
      901 aaaaaagtta tgtatcgaaa gatattacta taaaggatca gattcaaggt ggacagcagt
      961 tagatttaag cacattaaac attaatgtga caggtacaca tagcaattat tatagtggac
     1021 aaagtgcaat tactgatttt gaaa
//
