LOCUS       sequence-104788-        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of N-terminal fusion protein of CNA35 with 
            EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Aper SJ, van Spreeuwel AC, van Turnhout MC, van der Linden AJ, 
            Pieters PA, van der Zon NL, de la Rambelje SL, Bouten CV, Merkx M
  TITLE     Colorful Protein-Based Fluorescent Probes for Collagen Imaging.
  JOURNAL   PLoS One. 2014 Dec 9;9(12):e114983. doi: 
            10.1371/journal.pone.0114983. eCollection 2014.
  PUBMED    25490719
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-12-9"; volume: "9(12):e114983. doi"; pages: " 
            10.1371/journal.pone.0114983. eCollection 2014"
COMMENT     Sequence Label: pET28a-EGFP-CNA35
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagca
       61 atgccttaca ggtcgactga agccttggta tctttatcct gttttaaaac ttttaattca
      121 ccttttacag taccttcaat accggcatta gcattaatat tgtgcacagt atgattaaat
      181 gatttcccgt tcacttcttc cttaccatgc tcttgatacc aagcttgtga attattaaca
      241 aactcttttt gctgttcatt cgtaattttg gttttgtagt taattgaaaa actattatat
      301 gacccatagc cttgtggaat tgttacatca attgtgttct tcgtattatc aacagttatt
      361 ttagaacctg gaaaggcttt ttcaaaatca gtaattgcac tttgtccact ataataattg
      421 ctatgtgtac ctgtcacatt aatgtttaat gtgcttaaat ctaactgctg tccaccttga
      481 atctgatcct ttatagtaat atctttcgat acataacttt tttcattgtt aatatttaaa
      541 aaccatcgta catgtgtcgt atcttctggt agcatatctc ccgttttata atagaaaaca
      601 ctacttgttc ccgcttcact tttatgaacc gtaacattcg ttgatttatt cccagatgtt
      661 atcgtagcta ctttgtcatc tgaagtattt gtttgcgtta aatttcttcc ttgtacttca
      721 aattctgcaa atcccgaaac atcacttaat ttttctactt tatcattgaa tgtaattgtt
      781 gcaccgtctg gtgtaataac tgcttgaccc acctgttcac ctttaacagt taatggtact
      841 gttttactat aaccctctat ctttactgta ccgcttgtcg gccatgccac tttaatcatg
      901 tcaccatttt gtatttttcc atttttatcg tcgaacgtca tttttactgt cgttttacca
      961 ccatcttcta tcttagacgg tgatacagtt aaatctgtaa cattcgttga tgaaatatct
     1021 cgtgcggatc cgtggaattc tccggacttg tacagctcgt ccatgccga
//
