LOCUS       sequence-104807-       12321 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ubiquitous expression of a ""flp-Out"" Drosophila codon-optimized 
            GAL80 gene cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12321)
  AUTHORS   Nern A, Pfeiffer BD, Rubin GM
  TITLE     Optimized tools for multicolor stochastic labeling reveal diverse 
            stereotyped cell arrangements in the fly visual system.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 May 11. pii: 201506763.
  PUBMED    25964354
REFERENCE   2  (bases 1 to 12321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 May 11. pii: 201506763."
COMMENT     Sequence Label: tubP-FRT>GAL80-6-FRT>
FEATURES             Location/Qualifiers
     source          1..12321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    complement(2724..2757)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          2786..2852
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     misc_feature    4180..4768
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4233..4253)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4651..4662)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     intron          4862..4927
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5057..5077
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5349..5483
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5386..5405)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5440..5459
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(5501..5534)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(join(6844..7458,7529..7660,7864..8179,8241..8895,
                     8970..9243,9645..9716))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     10168..10186
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    10411..10480
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(10567..11155)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10647..10666)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11326..12117,12118..12186))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11949..11968
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12187..12291)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgatat caagcttgca caggtcctgt tcgataacgt cgtactcggg
      121 aatcaccttt aacttgtccg ctattgggta tttgtcctta aacacattcg taatctctcc
      181 cacaatcatt gccctggcct ctgcaatgta aaaagtattt ataaaatatc gctggctcta
      241 aaggaaacat attaatcaaa gtattgaggc tttatcaatc taacatttac aagtagtcag
      301 accacaattt ttagactgca cttctgcgta agtaaatgaa atgaaatggt gacctactct
      361 tgggtagccg tactgttata tgctgacgag ccttcacctg ggcctgcttt ttctcctgtt
      421 tcgtctgatg cgtcggatgc ggctggactg gctgattgtt gggattgggt tttggatgct
      481 gctccgaaag gtgggccaca gcttttgcga tggtggtgcc cactatgcgg ttgctgcagg
      541 aactacagct gaatgttcgc tttattgtct gcagctcctg gggctggacg aaggccacgc
      601 cctcacccgg tgcggccatc tcctgcggca aaacgtatgt gggattcctg tccaagtagc
      661 tctcgatgcc caccataatg cgctccagca ggttggccag gtgcgggtct agctgcggcg
      721 gattcgtcgt ctggttcact gagtttaggg tctgcagaaa gaagtgtgcc tccatggtaa
      781 gcggattcat ctgcacgatc agcgggcttt agtctaaaaa ttcgaatgat acaaactcgt
      841 actcacgtaa agcagtttgt ttggcatcat ctcggccatc gtcttattct tcttcgtggc
      901 ctttgttacc ggagcggcta tgttgggcgc ggtggagccc ggaagctgaa aggttaatcg
      961 aatgcgttaa cttttctgca actcgaaagt ttgccgcctt tgttcgactg ccaataactg
     1021 ttgattcgaa aattcgaatc gaagcggttg aatttcgtag ggtggccaac tacaccaaag
     1081 ttcgccggcg gtgtatgcat gtgagtgtga gtgtgacagt gggtgtacgt gtgctttaag
     1141 cgacacaatc gcaccgcgat gtacatattt ttgtagtaat gtgtgtgcat ttttcgtccg
     1201 gatcacggct ccataaaata aatcatatca cagggctcag ggatttgtga gtagaagttc
     1261 ccgccactta aacgtcactt gtgaaatcgc catcgacggt caacttttac tcacactggt
     1321 ctttcgcgga cggaccgtct caaagtactg cctttctgcg ttggaaaaca tcgccttttt
     1381 cgtccaaaag gagtccccag gttcgatccg catggcgttg tgcgtgcgtg cctttctttt
     1441 caaatgatta cggctattaa cttgggggcg ttaagttgga aacacgtaaa ttgcagactg
     1501 cgattagagt gaccatgagt aggagttcaa aatctcctga catcattttc ttaaaacctg
     1561 ctttgttttt tacatttcta tttaatataa ctcctatttg aataaaaaaa caaaacaagt
     1621 ttagatgtta agatattaac tacatccttt gctccaaagg gagaggggaa gttatggagt
     1681 taattaattt gctgttggaa atcaatatgg agtcagaaat ataatgattt actaaacctt
     1741 attgaatcgg taacgatgcg aatttatatt aaaatagctt ttatgaaaca ttcaacaaaa
     1801 atattattaa tgttggccca ctttagcaac cggttaggtc taccggttgg gcaagcaaag
     1861 attcacgccc tggttcgagt cccaactagt cctgcaaaat accgcagcaa gttttagaga
     1921 gaccaagtgc cattacctct cccacttcag ttatcggtta tgcggcgttt aagtcgacag
     1981 cttgccgtct ctagctccgg tgcctatata aagcagcccg ctttccacat ttcatattcg
     2041 ttttacgttt gtcaagcctc atagccggca gttcgaacgt atacgctctc tgagtcagac
     2101 ctcgaaatcg tagctctaca caattctgtg aattttcctt gtcgcgtgtg aaacacttcc
     2161 aataaaaact cctgcaggtg agtactttaa aaaaaaatct agtgaaataa tgctgaaaag
     2221 aaatttgtgt gggcaaaatt caatgggcaa aaacgcgatg cggctttttc tcaaaatggc
     2281 ggccggcctg cgttttttcc tcaaaagtga tgacgtcatg cctgtttttt tttttttgtt
     2341 cgcaatgagg aatggctctt aaaatctact agataaaaaa aatattcatt atttctatgc
     2401 tgctggaacg cttcattaat cttaaaaatt ctaaattcgg ttaccatgat acttcgacgc
     2461 ataactgtag attttggata gaattaaaga gaaaatggcg agagagtaaa attccggcgt
     2521 cggcaaagta gagcaaaaaa atcagtatac catttagcta cctctctcac tcgcacgcag
     2581 tgccggctca agttgggcgc ggctctgcaa ttatcgattt tcttggggtg tgtaactaat
     2641 catccgtttt cccttcctcc tcatccacag cgtgaagaat tcgtacatct agaataaacg
     2701 gccggccaga tctggtacca tctgaagttc ctatactttc tagagaatag gaacttctaa
     2761 cgtaagctag ctagaccggt aaaaggtagg ttcaaccact gatgcctagg cacaccgaaa
     2821 cgactaaccc taattcttat cctttacttc aggctagcaa tcaaaatgga ttacaacaaa
     2881 aggagtagtg tgagtacggt gccgaatgct gctcccattc gcgtgggctt cgtgggattg
     2941 aacgcggcta agggttgggc cattaaaacg cattatccag ccatactgca gctgagctcc
     3001 cagttccaaa taacagcgct ttattccccc aagatcgaga cgtccattgc gacaattcag
     3061 aggctgaagt tgtccaacgc cacagctttc ccaacactcg agagcttcgc ctcgagctcc
     3121 acgatcgata tgattgtgat cgcaatccag gtggcttccc actacgaggt ggtaatgccc
     3181 ctgctcgagt tttcgaagaa caatcctaat ctgaagtatt tgtttgtgga atgggcactg
     3241 gcctgctcgc tcgatcaggc ggagtcgatc tataaggccg ctgcagagcg gggcgtgcaa
     3301 acaataatca gtctgcaggg acgaaagagc ccgtacattt tgcgggcaaa agagcttatc
     3361 tcccagggct acatcggcga tataaatagc atcgagattg caggtaacgg aggttggtac
     3421 ggttacgagc ggccggtgaa aagcccgaaa tacatttacg agatcggaaa cggagttgat
     3481 ctggtgacca ccacgttcgg tcacacgata gatatattgc agtacatgac cagttcgtac
     3541 ttcagccgta tcaatgccat ggtgtttaac aacattccag aacaggagct cattgatgag
     3601 aggggcaatc gcctgggcca gcgggtccca aagactgtcc cagatcatct cttgttccag
     3661 ggcacgctgc tcaacggaaa tgttcctgtg tcctgttcct ttaagggagg caagcctacg
     3721 aaaaagttca ccaagaatct ggtcatagat atccatggta caaagggtga tctgaagctg
     3781 gagggtgacg ctggatttgc ggaaatctcg aatctcgttc tgtattattc gggcacccgc
     3841 gccaatgatt ttcccctggc caatggtcag caggcaccct tggaccccgg ctacgacgca
     3901 ggcaaggaga ttatggaggt gtatcacctc cgaaactaca acgcgatcgt tggtaatata
     3961 caccggctct accagagtat cagcgatttc cacttcaaca ccaagaaaat ccccgagctt
     4021 ccatcgcagt tcgtaatgca aggcttcgat ttcgaaggct ttcccaccct gatggatgcc
     4081 ttgatcttgc atcgcctgat tgaaagtgtc tacaagtcga atatgatggg cagtaccctg
     4141 aatgtttcca acatctccca ctactcgctt taaaagctta atcaacctct ggattacaaa
     4201 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4261 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4321 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4381 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4441 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4501 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4561 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     4621 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4681 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4741 cggatctccc tttgggccgc ctccccgcct gaagcttacg cgtaggatct ttgtgaagga
     4801 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     4861 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     4921 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     4981 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     5041 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     5101 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     5161 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattt
     5221 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
     5281 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     5341 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5401 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5461 atgtatctta tcatgtctgg atcactagtg atctggccgg gaagttccta tactttctag
     5521 agaataggaa cttcctcgag aataagctta tcgataccgt cgactaaagc caaatagaaa
     5581 attattcagt tcctggctta agtttttaaa agtgatatta tttatttggt tgtaaccaac
     5641 caaaagaatg taaataacta atacataatt atgttagttt taagttagca acaaattgat
     5701 tttagctata ttagctactt ggttaataaa tagaatatat ttatttaaag ataattgcgt
     5761 ttttattgtc agggagtgag tttgcttaaa aactcgttta gatccactag ttccctaggt
     5821 cgtgctagct cgtgcggccg ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg
     5881 acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag
     5941 cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc
     6001 ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg
     6061 ttccggaggc gcgccctagt tccagtgaaa tccaagcatt ttctaaatta aatgtattct
     6121 tattattata gttgttattt ttgatatata taaacaacac tattatgccc accatttttt
     6181 tgagatgcat ctacacaagg aacaaacact ggatgtcact ttcagttcaa attgtaacgc
     6241 taatcactcc gaacaggtca caaaaaatta ccttaaaaag tcataatatt aaattagaat
     6301 aaatatagct gtgagggaaa tatatacaaa tatattggag caaataaatt gtacatacaa
     6361 atatttatta ctaatttcta ttgagacgaa atgaaccact cggaaccatt tgagcgaacc
     6421 gaatcgcgcg gaactaacga cagtcgctcc aaggtcgtcg aacaaaaggt gaatgtgttg
     6481 cggagagcgg gtgggagaca gcgaaagagc aactacgaaa cgtggtgtgg tggaggtgaa
     6541 ttatgaagag ggcgcgcgat ttgaaaagta tgtatataaa aaatatatcc cggtgtttta
     6601 tgtagcgata aacgagtttt tgatgtaagg tatgcaggtg tgtaagtctt ttggttagaa
     6661 gacaaatcca aagtctactt gtggggatgt tcgaagggga aatacttgta ttctataggt
     6721 catatcttgt ttttattggc acaaatataa ttacattagc tttttgaggg ggcaataaac
     6781 agtaaacacg atggtaataa tggtaaaaaa aaaaaacaag cagttatttc ggatatatgt
     6841 cggctactcc ttgcgtcggg cccgaagtct tagagccaga tatgcgagca cccggaagct
     6901 cacgatgaga atggccagac ccacgtagtc cagcggcaga tcggcggcgg agaagttaag
     6961 cgtctccagg atgaccttgc ccgaactggg gcacgtggtg ttcgacgatg tgcagctaat
     7021 ttcgcccggc tccacgtccg cccattggtt aatcagcaga ccctcgttgg cgtaacggaa
     7081 ccatgagagg tacgacaacc atttgaggta tactggcacc gagcccgagt tcaagaagaa
     7141 gccgccaaag agcaggaatg gtatgataac cggcggaccc acagacagcg ccatcgaggt
     7201 cgaggagctg gcgcaggata ttagatatcc gaaggacgtt gacacattgg ccaccagagt
     7261 gaccagcgcc aggcagttga agaagtgcag cactccggcc cgcagtccga tcatcggata
     7321 ggcaatcgcc gtgaagacca gtggcactgt gagaaaaagc ggcaattcgg caatcgtttt
     7381 gcccagaaag tatgtgtcac agcgataaag tcgacttcgg gcctccctca taaaaactgg
     7441 cagctctgag gtgaacacct aaatcgaatc gattcattag aaagttagta aattattgaa
     7501 atgcaaatgt attctaaaca tgacttacat ttatcgtggc aaagacgttt tgaaaggtca
     7561 tgttggtcag gaagaggaag atggctccgt tgatattcat cacacccact tgcgtgagtt
     7621 gttggcccaa aaagatgagg ccaatcaaga tggcaaccat ctgcaaatta aaatgttact
     7681 cgcatctcat taatattcgc gagttaaatg aaatttattt atcttctgca aaactataaa
     7741 ctatacatct cattgaaaaa aactaagaag ggtgtggaat caggcaattc tatctaaaat
     7801 ctagcgaatt tgtttccaag aattgtaagc gttatatcat ttgtttccac tggaaccact
     7861 caccgttgtc tgaataagtc gcacttttac gaggagtggt tccttgagca ccgacagcca
     7921 ggatcgccac aggaccgccc ggaactgcat gaaccaggtg gccttgtagg tgtacccatt
     7981 ctccggctgc tccagtggct tctccagatt tttggtggcc aacaactgct ccatatcccg
     8041 ggctactttg ctaatggcaa aattgtcgca tatcttggcg atccgatcac gggactcgat
     8101 ctcccgtccg ggcacaacgg ccaacacctg tacgtaaaag tccgccggat tgtagttggt
     8161 aggacactgg gcacccacgc tggataggag ttgagatgtt atgtaatact agataccctt
     8221 aataaacaca tcgaactcac taggaaaaga agtcgacggc ttcgctggga gtgcccaaga
     8281 aagctaccct gccctcggcc atcagaagga tcttgtcaaa gagctcaaac agctcggaag
     8341 acggctgatg aatggtcagg atgacggtct tgcccttctg cgacagcttc ttcagcacct
     8401 ggacgacgct gtgggcggta aaggagtcca gtccggaggt gggctcatcg cagatcagaa
     8461 gcggcggatc ggttagagcc tcggaggcga atgccagacg cttcctttct ccgccggaca
     8521 gacctttcac cctgccgggc acaccgatga tcgtgtgctg acatttgctg agcgaaagct
     8581 cctggatcac ctgatccacg cgggccactc gctgccgata ggtcagatgt cgtggcatcc
     8641 gcaccatggc ttggaaaatc aggtgttccc tggccgttag ggagccgata aagaggtcat
     8701 cctgctggac ataggcgcac ctggcctgca tctccttggc gtccacaggt tggccattga
     8761 gcagtcgcat cccggatggc gatacttgga tgccctgcgg cgatcgaaag gcaagggcat
     8821 tcagcagggt cgtctttccg gcaccggaac tgcccatcac ggccaaaagt tcgcccggat
     8881 aggccacgcc gcaaactgag tttcaaattg gtaattggac cctttattaa gatttcacac
     8941 agatcagccg actgcgaata gaaactcacc gttcttgagc aaatgtttcc tgggcgccgg
     9001 tatgtgtcgc tcgttgcaga atagtccgcg tgtccggttg accagctgcc gccatccgga
     9061 gcccggctga ttgaccgccc caaagatgtc catattgtgc caggcatagg tgaggttctc
     9121 ggctagttgg ccgctccctg aaccggagtc ctccggcgga ctgggtggcc ggagcgtgcc
     9181 gtagtttttg gcctgcccga agccctggtt aatgcagctc tgcgaagccg ctccgctgtc
     9241 accctgcaat gataggggat ctcaaatatc aactactagc gttatgctca tctaaccccg
     9301 aacaaaaagt accccgaagt atcctacgaa gtaggtttat acttttattt attttttgtg
     9361 catctaggat cagcttaaaa tatctggttg ttatattttt tgtaaaaaag aatatagtcg
     9421 aaaatgaatg cctttagatg tcttgatcat gatatgatct caaaaattgt cttatatagc
     9481 gagaacagct accagaataa tctgtttcgt gtcactattt gtttgtgcaa ttgcggtttg
     9541 ggatttttgt gggtcgcagt tctcacgccg catacaattt gatgttgcaa tcgcagttcc
     9601 tatagatcaa gtgaacttaa gatgtatgca catgtactac tcacattgtt cagatgctcg
     9661 gcagatgggt gtttgctgcc tccgcgaatt aatagctcct gatcctcttg gcccattgcc
     9721 gggatttttc acactttccc ctgcttaccc acccaaaacc aatcaccacc ccaatcactc
     9781 aaaaaacaaa caaaaataag aagcgagagg agttttggca cagcactttg tgtttaattg
     9841 atggcgtaaa ccgcttggag cttcgtcacg aaaccgctga caaaatgcaa ctgaaggcgg
     9901 acattgacgc tacgtaacgc tacaaacggt ggcgaaagag atagcggacg cagcggcgaa
     9961 agagacggcg atatttctgt ggacagagaa ggaggcaaac agcgctgact ttgagtggaa
    10021 tgtcattttg agtgagaggt aatcgaaaga acctggtaca tcaaataccc ttggatcgaa
    10081 gtaaatttaa aactgatcag ataagttcaa tgatatccag tgcagtaaaa aaaaaaaatg
    10141 ttttttttat ctactttccg caaaaatggg ttttattaac ttacatacat actaggcgcg
    10201 cccatatgtt cggcttgtcg acatgcccgc cgtgaccgtc gagaacccgc tgacgctgcc
    10261 ccgcgtatcc gcacccgccg acgccgtcgc acgtcccgtg ctcaccgtga ccaccgcgcc
    10321 cagcggtttc gagggcgagg gcttcccggt gcgccgcgcg ttcgccggga tcaactaccg
    10381 ccacctcgac ccgttcatca tgatggacca gatgggtgag gtggagtacg cgcccgggga
    10441 gcccaagggc acgccctggc acccgcaccg cggcttcgag accgtgacct acatcgtcga
    10501 cggtaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    10561 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    10621 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    10681 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    10741 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    10801 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    10861 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    10921 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    10981 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    11041 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    11101 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    11161 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    11221 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    11281 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    11341 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    11401 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    11461 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    11521 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    11581 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    11641 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    11701 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    11761 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    11821 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    11881 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    11941 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    12001 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    12061 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    12121 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    12181 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    12241 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    12301 ccgaaaagtg ccacctgagt c
//
