LOCUS       sequence-104825-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used to characterize the promoter activities of PTet for dCas9 and 
            sgRNA response functions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Nielsen AA, Voigt CA
  TITLE     Multi-input CRISPR/Cas genetic circuits that interface host 
            regulatory networks.
  JOURNAL   Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
  PUBMED    25422271
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-24"; volume: "10:763. doi"; pages: " 
            10.15252/msb.20145735"
COMMENT     Sequence Label: pAN-PTet-YFP
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(15..64)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     protein_bind    77..95
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    102..120
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(287..306)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(371..390)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 attatttgta gaggctgttt cgtcctcacg gactcatcag accggaaagc acatccggtg
       61 acagctgtgc tcattatctc tatcactgat agggatgtca atctctatca ctgataggga
      121 aaaaagccaa cggtggagta aagcgggaga ccagaaacaa aaaaaggccc cccgttaggg
      181 aggccttcaa taattggttg tgtctcaaaa tctctgatgt acattgcaca agataaaaat
      241 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
      301 agccatattc aacgggaaac gtcttgctcc aggccgcgat taaattccaa catggatgct
      361 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
      421 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
      481 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
      541 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
      601 cccgggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
      661 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
      721 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
      781 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaa
//
