LOCUS       sequence-104832-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human immunoglobulin epsilon antibody expression vector (kappa light
            chain) without variable regions. Enables Colony-PCR screening of 
            false-positive (PCR vector template) colonies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Dodev TS, Karagiannis P, Gilbert AE, Josephs DH, Bowen H, James LK, 
            Bax HJ, Beavil R, Pang MO, Gould HJ, Karagiannis SN, Beavil AJ
  TITLE     A tool kit for rapid cloning and expression of recombinant 
            antibodies.
  JOURNAL   Sci Rep. 2014 Jul 30;4:5885. doi: 10.1038/srep05885.
  PUBMED    25073855
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-07-30"; volume: "4:5885. doi"; pages: " 
            10.1038/srep05885"
COMMENT     Sequence Label: pVITRO1-dV-IgE/kappa
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaggtatctt ttaaaccctt ttttaggtgt tgtgaaaacc accgctaatt caaagcaacc
       61 ggtatggact ggacctggag gatcctcttc ttggtggcgg ccgccacagg cgcgcactcc
      121 gaattcgata tcctcgaggc tagcacacag agcccatccg tcttcccctt gacccgctgc
      181 tgcaaaaaca ttccctccaa tgccacctcc gtgactctgg gctgcctggc cacgggctac
      241 ttcccggagc cggtgatggt gacctgggac acaggctccc tcaacgggac aactatgacc
      301 ttaccagcca ccaccctcac gctctctggt cactatgcca ccatcagctt gctgaccgtc
      361 tcgggtgcgt gggccaagca gatgttcacc tgccgtgtgg cacacactcc atcgtccaca
      421 gactgggtcg acaacaaaac cttcagcgtc tgctccaggg acttcacccc gcccaccgtg
      481 aagatcttac agtcgtcctg cgacggcggc gggcacttcc ccccgaccat ccagctcctg
      541 tgcctcgtct ctgggtacac cccagggact atcaacatca cctggctgga ggacgggcag
      601 gtcatggacg tggacttgtc caccgcctct accacgcagg agggtgagct ggcctccaca
      661 caaagcgagc tcaccctcag ccagaagcac tggctgtcag accgcaccta cacctgccag
      721 gtcacctatc aaggtcacac ctttgaggac agcaccaaga agtgtgcaga ttccaacccg
      781 agaggggtga gcgcctacct aagccggccc agcccgttcg acctgttcat ccgcaagtcg
      841 cccacgatca cctgtctggt ggtggacctg gcacccagca aggggaccgt gaacctgacc
      901 tggtcccggg ccagtgggaa gcctgtgaac cactccacca gaaaggagga gaagcagcgc
      961 aatggcacgt taaccgtcac gtccaccctg
//
